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Identification of a disulfide bonded protein from cytoplasm of Bacillus subtilis

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Chinese Science Bulletin 1997年第20期42卷 1756-1760页

作者： Wang, T Kadokura, H Yamasaki, M Chinese Acad Sci Inst Bot Beijing 100095 Peoples R China

DISULFIDE bonds are important for some proteins to form stable secondary or tertiary conforma-tion. These known disulfide bonded proteins are mainly present in periplasm or cel1ular or-ganelles, most of which are secreted proteins or membrane proteins. It is generally recog-nized that cytoplasmic proteins do not have disulfide bonds because of reducing environment

关键词： Bacillus subtilis cytoplasm disulfide bonded protein.

An AFM Observation on Fossil cytoplasm

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Acta Geologica Sinica(English Edition) 2008年第6期82卷 1141-1145页

作者： WANG Xin YU Junping FANG Xiaohong State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and Palaeontology Nanjing 210008 China Key Laboratory of Molecular Nanostructure and Nanotechnology Institute of Chemistry Beijing 100080 China

Fossil cytoplasm is a new research topic of interest in paleobotany. Atomic force microscope （AFM） is a new technology applied widely in physics and biology; however, it is rarely used in paleontology. Here we applied AFM for the first time to study fossil cytoplasm. The results indicate that the fossil cytoplasm is heterogeneous and full of ultrastructures, just like extant cytoplasm, and that the application of AFM, especially in combination with other techniques, can reveal the subcellular details of fossil plants with more confidence.

关键词： cytoplasm fossil AFM plant

Breeding of wheat male sterile line with Aegilops crassa (6x) cytoplasm and research of its characters

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Chinese Science Bulletin 1995年第3期40卷 243-247页

作者：吴郁文张翠兰刘春光任树新张炎 State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics Chinese Academy of Sciences Beijing 100101 China

Since Wilson and Ross （1962） bred wheat male sterile line with T. timopheevi cytoplasm （G type） and its relative restoration line, with high utilization value, the progress has been accelerated in research of heterotic use of wheat male sterility. But so far they have not been used in large area production, because of the limited restorer resource, imperfect grains and insignificant population heterosis. Ae（?）ilops kotschyi and Ae. Variabilis types/（Sv type） overcame well some problems in G type, such as restorer resource, grain plumpness etc., but at the same time, other more difficult problems arose, e. g. their sterile line and hybrid F1 generation produced monoploids at a certain rate, hybrid growth was weak and restorer degree of sterility had a large variation scale. The stability of sterility in Ae. Ventricosa （D type） and Ae unianstata （Mu type） cytoplasms, as well as the maintenance and restoration of their male sterile lines were further studied. In order

关键词： Aegilops crassa (6x) male sterility restoration cytoplasm effect.

cytoplasm Has No Effect on the Yield and Quality of Biomass Sorghum Hybrids

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Journal of Sustainable Bioenergy Systems 2013年第2期3卷 129-134页

作者： Leo Hoffmann Jr. William L. Rooney Department of Soil and Crop Sciences Texas A & M University College Station USA

The development of the biomass sorghum hybrid seed industry is contingent on the use of cytoplasmic male sterility. Within sorghum, there are several different cytoplasmic male sterility systems and it is important to determine early in development if cytoplasm will affect agronomic performance or composition characters. Thus, if there is a difference, then the best system can be deployed. The purpose of this study was to determine if cytoplasm per se influences agronomic performance of biomass sorghum using a set of iso-cytoplasmic hybrids. Three hybrid genotypes were produced in three different cytoplasms (A1, A2, and A3) for a total of nine hybrids. These hybrids were evaluated for plant height, biomass yield, and biomass composition in three Texas environments (Weslaco, College Station, and Halfway) in 2010. Across environments, significant differences existed among hybrids for both agronomic and compositional traits, but cytoplasm per se had no effect on any measured trait. Since cytoplasm did not effect on hybrid performance, any of the tested cytoplasms (A1, A2, and A3) can be deployed in hybrid biomass sorghums.

关键词： Near Infrared Spectroscopy Photoperiod Sensitive cytoplasm

Expression of human transforming growth factor β1 in E. coli (I) --Direct expression in cytoplasm

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Science China(Life Sciences) 1998年第5期41卷 548-554页

作者：龙建银王会信刘莉王芳周廷冲 Beijing Institute of Basic Medical Sciences China

PCR technique is used to amplify the mature peptide gene of human transforming growth factor β1(hTGFβ1); the gene is verified by full length sequence analysis. In DH5α/pBV220 expression system, hTGFβ1 attains expression in the cytoplasm of \%E. coli\% up to 16%. The recombinant protein is proved to be the monomer of hTGFβ1 by N terminal amino acids analysis and immunoblotting. After refolding of the monomer protein \%in vitro\% in glutathione system or CHPAS/DMSO system, the dimeric protein accumulates to 30% in the refolding mixture. The recombinant protein is purified to homogeneity on silver staining, and is shown to have strong biological activity from MTT bioassay on Mv1Lu cells.

关键词： TGFβ1 E. coli expression in cytoplasm assembly and renaturation in vitro.

Teaching Design of“cytoplasm”(the First Class)

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Journal of Contemporary Educational Research 2018年第1期2卷 29-33页

作者： Chen Yangyi Taizhou Luqiao Middle School 318050

Taking the"synthesis and secretion processes of secreted proteins"as the main chain and with the teaching method of"identifying graphsdiscussion-drawing-display-evaluation",this class is designed to guide students to understand the organelles related to the formation of secreted *** helps students to understand that the organelles in the cytoplasm are not independent and incoherent,but a coordinated unity with the division of labor.

关键词： cytoplasm secreted proteins teaching design

FORCING INTERCELLULAR cytoplasm AND CHROMATIN MIGRATION BETWEEN THE MERISTEMTIC CELLS OF Pinellia ternate AND Commelina communis BY LB TECH NIQUE AND THE SUBSEQUENT REGENERATION OF HYBRID PLANTS

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Chinese Science Bulletin 1992年第4期37卷 350-350页

作者：崔亚亚陈毅平吴伯骥 Chengdu Institute of Biology Academia Sinica Chengdu 610015 PRC

The interfamilical hybrid plants with color character for genetic marker of Pinellia ternate and Commelina communis have been obtained successfully by the LB technique——forcing intercellular cytoplasm and chromatin migration between cells of different plants by artificial motive force in our lab. The preliminary results are as follows.

关键词： Pinellia ternate communis intercellular migration motive preliminary cytoplasm marker osmotic

cytoplasmic and nuclear genome variations of rice hybrids and their parents inform the trajectory and strategy of hybrid rice breeding

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Molecular Plant 2021年第12期14卷 2056-2071页

作者： Zhoulin Gu Zhou Zhu Zhen Li Qilin Zhan Qi Feng Congcong Zhou Qiang Zhao Yan Zhao Xiaojian Peng Bingxin Dai Rongrong Sun Yan Li Hengyun Lu Lei Zhang Tao Huang Junyi Gong Danfeng Lv Xuehui Huang Bin Han National Center for Gene Research State Key Laboratory of Plant Molecular GeneticsCenter for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200233China University of Chinese Academy of Sciences Beijing 100049China College of Life Sciences Anhui Normal UniversityWuhuAnhui 241000China School of Life Sciences Anhui Agricultural UniversityHefeiAnhui 230036China School of Life Science and Technology Shanghai Tech UniversityShanghai 201210China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 310006China College of Life Sciences Shanghai Normal UniversityShanghai 200234China

The male sterility(MS)line is a prerequisite for efficient production of hybrid seeds in rice,a self-pollinating *** line breeding is pivotal for hybrid rice *** the historical breeding tra-jectory will help to improve hybrid rice breeding *** inherited cytoplasm is an appro-priate tool for phylogenetic reconstruction and pedigree tracing in rice *** this study,we analyzed the cytoplasmic genomes of 1495 elite hybrid rice varieties and identified five major types of cytoplasm,which correspond to different hybrid production *** the cytoplasm donors for hybrids,461 MS lines were also divided into five major types based on cytoplasmic and nuclear genomic ***-cific core accessions cooperating with different fertility-associated genes drove the sequence divergence of MS *** to hundreds of convergent and divergent selective sweeps spanning several agro-nomic trait-associated genes were identified among different types of MS *** further analyzed the cross patterns between different types of MS lines and their corresponding *** study system-atically analyzed the cytoplasmic genomes of rice hybrids revealed their relationships with nuclear ge-nomes of MS lines,and illustrated the trajectory of hybrid rice breeding and the strategies for breeding different types of MS lines providing new insights for future improvement of hybrid rice.

关键词： rice hybrid cytoplasm cytoplasmic male sterile environment-sensitive genic male sterile breeding

cytoplasmic Ca2＋ Dynamics under the Interplay between the Different IP3R Gating Models and the Plasma Membrane Ca2＋ Influx

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Chinese Physics Letters 2010年第1期27卷 60-63页

作者：陈晓芳李丛鑫王鹏业王渭池 Laboratory of Soft Matter Physics Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190

The cytoplasmic Ca2＋ oscillations are investigated under the effect of CRAC channels in non-excitable cells （especially in T cells）. The oscillatory Ca2＋ signals can be modulated as the amplitude-, frequency- and mixed amplitude-frequency modulation modes dependent on the different IP3R gating models. Bifurcation analyses show that Ca2＋ signals in the single positive feedback loop model is a mixed modulation mode. In contrast, Ca2＋ signals in the Mak-McBride-Foskett model demonstrates approximately the frequency modulation mode only with slight amplitude shifts.

关键词： CALCIUM ions cytoplasm CELL membranes PHOSPHATES OSCILLATING chemical reactions AMPLITUDE modulation BIFURCATION theory MATHEMATICAL models