NSUN2-mediated mRNA m^(5)C Modification Regulates the Progression of Hepatocellular Carcinoma

作     者：Dan Song Ke An Wenlong Zhai Luyao Feng Yingjie Xu Ran Sun Yueqin Wang Yun-Gui Yang Quancheng Kan Xin Tian 

作者机构：Department of Pharmacythe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China CAS Key Laboratory of Genomic and Precision MedicineBeijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China Henan Key Laboratory of Precision Clinical PharmacyZhengzhou UniversityZhengzhou 450052China Department of Hepatobiliary and Pancreatic Surgerythe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Digestive Organ Transplantation of Henan ProvinceZhengzhou UniversityZhengzhou 450052China 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2023年第21卷第4期

页      面：823-833页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Grant Nos.32170594 and 31870809) the Province and Ministry Coconstruction Major Program of Medical Science and Technique Foundation of Henan Province(Grant No.SBGJ202001007) the Special Fund for Young and Middle School Leaders of Henan Health Commission(Grant No.HNSWJW-2020017),China 

主　　题：5-methylcytosine Hepatocellular carcinoma NSUN2 Ras pathway Sorafenib 

摘      要：RNA modifications affect many biological processes and physiological *** 5-methylcytosine(m^(5)C)modification regulates the progression of multiple ***,its characteristics and functions in hepatocellular carcinoma(HCC)remain largely ***,we found that HCC tissues had a higher m^(5)C methylation level than the adjacent normal *** analysis revealed that the hypermethylated genes mainly participated in the phosphokinase signaling pathways,such as the Ras and PI3K-Akt *** m^(5)C methyltransferase NSUN2 was highly expressed in HCC ***,the expression of many genes was positively correlated with the expression of NSUN2,including GRB2,RNF115,AATF,ADAM15,RTN3,and ***-time PCR assays further revealed that the expression of the mRNAs of GRB2,RNF115,and AATF decreased significantly with the down-regulation of NSUN2 expression in HCC ***,NSUN2 could regulate the cellular sensitivity of HCC cells to sorafenib via modulating the Ras signaling ***,knocking down NSUN2 caused cell cycle *** together,our study demonstrates the vital role of NSUN2 in the progression of HCC.