Selenium reduces hepatopancreas lipid accumulation of grass carp(Ctenopharyngodon idella)fed high-fat diet via lipophagy activation

作     者：Xiaotian Zhang Haibo Yu Xianfang Yan Pengju Li Chi Wang Cheng Zhang Hong Ji Xiaotian Zhang;Haibo Yu;Xianfang Yan;Pengju Li;Chi Wang;Cheng Zhang;Hong Ji

作者机构：College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第15卷第4期

页      面：126-136页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学] 

基　　金：This research was funded by Key Research and Development Project of Shaanxi Province(2023-YBNY-116) National Natural Science Foundation of China(31602180) 

主　　题：Grass carp Selenium High-fat diet Lipid metabolism Lipophagy 

摘      要：It has been reported that selenium(Se)can reduce hepatopancreas lipid accumulation induced by highfat ***,its mechanism is still *** study aims to investigate the specific mechanisms by which Se alleviates high-fat diet-induced lipid *** carp were fed control diet(4.8%lipid,Con),high-fat diet(8.8%lipid,HFD)or HFD supplemented with 0.3 mg/kg nano-Se(HSe0.3)for *** performance,Se deposition,lipid accumulation,hepatic ultrastructure,and gene and protein expression levels associated with autophagy were ***,oleic acid(OA)was used to incubate the grass carp hepatocytes(L8824)for 24 h,and then the L8824 were incubated with sodium selenite in presence or absence of an autophagy inhibitor for 24 h.L8824 was analyzed for triglyceride concentration,immunofluorescence,and gene and protein expression levels associated with *** found that dietary nano-Se improved the growth of fish fed HFD and also decreased hepatosomatic index and intraperitoneal fat ratio of fish fed HFD(P0.05).HFD significantly increased hepatopancreas lipid accumulation and decreased autophagic activity(P0.05).Treatment of grass carp fed HFD with nano-Se decreased lipid accumulation and restored hepatic autophagy(P0.05).In vitro,Se(100μM sodium selenite)obviously activated autophagy in L8824 incubated with OA,and consequently reduced the lipid accumulation induced by OA(P0.05).Furthermore,using pharmacological inhibition(chloroquine)of the autophagy greatly diminished the beneficial effects of Se on alleviating OA-induced lipid accumulation and increased the co-localization of lipid droplets with autophagosome(P0.05),which indicated that Se increased autophagic *** conclusion,these results suggest that Se alleviates HFD-induced hepatopancreas lipid accumulation by activating lipophagy.