Nerve growth factor promotes in vitro proliferation of neural stem cells from tree shrews

作     者：Liu-lin Xiong Zhi-wei Chen Ting-hua Wang 

作者机构：Department of AnesthesiologyInstitute of Neurological DiseaseTranslational Neuroscience CenterWest China HospitalSichuan University Institute of NeuroscienceKunming Medical University Key Laboratory of National Physical Fitness and Altitude Training Adaptation in Yunnan Province Institute of Physical EducationYunnan Normal University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第4期

页      面：591-596页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：supported by a grant from the National Key Technology Research and Development Program of the Ministry of Science and Technology of China No.2014BAI01B00 

主　　题：nerve regeneration tree shrews hippocampus neural stem cells cell proliferation nerve growth factor neurosphere embryo cell number cell therapy in vitro neural regeneration 

摘      要：Neural stem cells promote neuronal regeneration and repair of brain tissue after injury,but have limited resources and proliferative ability in *** hypothesized that nerve growth factor would promote in vitro proliferation of neural stem cells derived from the tree shrews,a primate-like mammal that has been proposed as an alternative to primates in biomedical translational *** cultured neural stem cells from the hippocampus of tree shrews at embryonic day 38,and added nerve growth factor（100 μg/L） to the culture *** stem cells from the hippocampus of tree shrews cultured without nerve growth factor were used as *** 3 days,fluorescence microscopy after DAPI and nestin staining revealed that the number of neurospheres and DAPI/nestin-positive cells was markedly greater in the nerve growth factor-treated cells than in control *** findings demonstrate that nerve growth factor promotes the proliferation of neural stem cells derived from tree shrews.