Expression of miR‑138 in cryopreserved bovine sperm is related to their fertility potential

作     者：Albert Salas‑Huetos Jordi Ribas‑Maynou Yentel Mateo‑Otero Carolina Tamargo Marc Llavanera Marc Yeste Albert Salas-Huetos;Jordi Ribas-Maynou;Yentel Mateo-Otero;Carolina Tamargo;Marc Llavanera;Marc Yeste

作者机构：Unit of Cell BiologyDepartment of BiologyFaculty of SciencesUniversity of GironaGirona 17003Spain. Biotechnology of Animal and Human Reproduction(TechnoSperm)Institute of Food and Agricultural TechnologyUniversity of GironaGirona 17003Spain. Department of NutritionHarvard T.H.Chan School of Public HealthHarvard UniversityBostonMA 02115USA. Consorcio CIBERM.P.Fisiopatologia de la Obesidad y Nutricion(CIBERobn)Instituto de Salud Carlos III(ISCIII)Madrid 28029Spain. Present Address:Unit of Preventive Medicine and Public HealthFaculty of Medicine and Health SciencesUniversitat Rovira i VirgiliReus 43201Spain. Department of Animal Selection and ReproductionThe Regional Agri-Food Research and Development Service of Asturias(SERIDA)Gijon 33394Spain. Catalan Institution for Research and Advanced Studies(ICREA)Barcelona 08010Spain. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2023年第14卷第6期

页      面：2383-2394页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：the Ministry of Science and Innovation,Spain(IJC2019-039615-I and PID2020-113320RB-I00) the European Union’s Horizon 2020 research and innovation scheme under the Marie Sklodowska-Curie grant agreement No.801342(Techniospring INDUSTRY TECSPR-19-1-0003) the Regional Government of Catalonia(2017-SGR-1229 and 2021-SGR-00900) the Catalan Institution for Research and Advanced Studies(ICREA) 

主　　题：Bovine Fertility miRNAs Sperm 

摘      要：Background MicroRNAs(miRNAs)are small,single-stranded,non-coding RNA molecules of 22–24 nucleotides that regulate gene *** the last decade,miRNAs have been described in sperm of several mammals,including *** is known that miRNAs can act as key gene regulators of early embryogenesis in mice and humans;however,little is known about the content,expression,and function of sperm-borne miRNAs in early bovine *** this study,total sperm RNA was isolated from 29 cryopreserved sperm samples(each coming from a separate bull)using a RNeasy kit and treatment with DNase *** concentration and purity were determined through an Epoch spectrophotometer and an Agilent *** expression of 10 candidate miRNAs in bovine sperm(bta-miR-10a,bta-miR-10b,bta-miR-138,bta-miR-146b,bta-miR-19b,bta-miR-26a,bta-miR-34a,bta-miR-449a,bta-miR-495 and btamiR-7),previously identified in testis and/or epididymis,was evaluated with *** cel-miR-39-3p was used as a spike-in exogenous *** Mann–Whitney tests were run to evaluate which miRNAs were differentially expressed between bulls with high fertility[HF;non-return rates(NRR)ranging from 39.5 to 43.5]and those with subfertility(SF;NRR ranging from 33.3 to 39.3).Several sperm functionality parameters(e.g.,viability,membrane stability or oxygen consumption,among others)were measured by multiplexing flow cytometry and oxygen sensing *** RNA concentration and purity(260/280 nm ratio)(mean±SD)from the 29 samples were 99.3±84.6 ng/μL and 1.97±0.72,*** results confirmed the lack of RNA from somatic *** terms of the presence or absence of miRNAs,and after applying the Livak method,8 out of 10 miRNAs(bta-miR-10b,-138,-146b,-19b,-26a,-449a,-495,-7)were consistently detected in bovine sperm,whereas the other two(bta-miR-10a,and-34a)were ***,the relative expression of one miRNA(bta-miR-138)in sperm was significantly lower in the SF than in the HF gr