The WAK-like protein RFO1 acts as a sensor of the pectin methylation status in Arabidopsis cell walls to modulate root growth and defense

作     者：Apolonio I.Huerta Gloria Sancho-Andre´s Juan Carlos Montesinos Javier Silva-Navas Sole`ne Bassard Corinne Pau-Roblot Christopher Kesten Rudolf Schlechter Susanne Dora Temurkhan Ayupov Je´rome Pelloux^ Julia Santiago Clara Sa´nchez-Rodrı´guez Apolonio I.Huerta;Gloria Sancho-Andrés;Juan Carlos Montesinos;Javier Silva-Navas;Solène Bassard;Corinne Pau-Roblot;Christopher Kesten;Rudolf Schlechter;Susanne Dora;Temurkhan Ayupov;Jér?me Pelloux;Julia Santiago;Clara Sánchez-Rodríguez

作者机构：ETH ZurichInstitute of Molecular Plant Biology(D-BIOL)ZurichSwitzerland University of LausanneDepartment of Plant Molecular BiologyLausanneSwitzerland UMRT INRAE 1158 BioEcoAgro–BIOPI Biologie des Plantes et InnovationUniversite´de Picardie33 Rue St Leu80039 AmiensFrance Present address:Lonza AGVispVisSwitzerland Present address:Institute of MicrobiologyFreie Universitat BerlinBerlinGermany Present address:Institute of Molecular and Clinical Ophthalmology-BaselBaselSwitzerland Present address:Centro de Biotecnologı´a y Geno´mica de PlantasUniversidad Polite´cnica de Madrid(UPM)–Instituto Nacional de Investigacio´n y Tecnologı´a Agraria y Alimentaria(INIA/CSIC)Campus de Montegancedo UPM28223 Pozuelo de Alarco´nSpain 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2023年第16卷第5期

页      面：865-881页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the Swiss National Foundation and the Heinz Imhof Foundation to C.S.R.(2-72160-16 to A.I.H.,SNF 31003A_163065/1 and SNF 310030_184769 to G.S.) the Peter und Traudl Engelhorn Stiftung to C.K.,and the European Research Council(ERC)grant agreement no.716358 the Fondation Philanthropique Famille Sandoz to J.S. 

主　　题：alterations walls pectin 

摘      要：Most organisms adjust their development according to the environmental conditions.For the majority,this implies the sensing of alterations to cell walls caused by different cues.Despite the relevance of this process,few molecular players involved in cell wall sensing are known and characterized.Here,we show that the wall-associated kinase-like protein RESISTANCE TO FUSARIUM OXYSPORUM 1(RFO1)is required for plant growth and early defense against Fusarium oxysporum and functions by sensing changes in the pectin methylation levels in the cell wall.The RFO1 dwell time at the plasma membrane is affected by the pectin methylation status at the cell wall,regulating MITOGEN-ACTIVATED PROTEIN KINASE and gene expression.We show that the extracellular domain of RFO1 binds de-methylated pectin in vitro,whose distribution in the cell wall is altered during F.oxysporum infection.Further analyses also indicate that RFO1 is required for the BR-dependent plant growth alteration in response to inhibition of pectin de-methyl-esterase activity at the cell wall.Collectively,our work demonstrates that RFO1 is a sensor of the pectin methylation status that plays a unique dual role in plant growth and defense against vascular pathogens.