可去除染料--N-聚糖多方法深入分析中的缺失环节

作     者：Samanta Cajic RenéHennig Valerian Grote Udo Reichl Erdmann Rapp Samanta Cajic;René Hennig;Valerian Grote;Udo Reichl;Erdmann Rapp

作者机构：Max Planck Institute for Dynamics of Complex Technical SystemsMagdeburg 39106Germany glyXera GmbHMagdeburg 39120Germany Otto-von-Guericke UniversityChair of Bioprocess EngineeringMagdeburg 39106Germany 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2023年第26卷第7期

页      面：132-150,I0006页

核心收录：

学科分类：0810[工学-信息与通信工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0808[工学-电气工程] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 10[医学] 

基　　金：support from the German Federal Ministry of Education and Research(BMBF)under the project“Die Golgi Glykan Fabrik 2.0”(031A557C for Samanta Cajic and Erdmann Rapp) the European Commission(EC)under the project“HighGlycan”(278535 for RenéHennig and Erdmann Rapp) the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)under the project“The concert of dolicholbased glycosylation:from molecules to disease models”(FOR2509 for Valerian Grote and Erdmann Rapp) 

主　　题：Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary gel electrophoresis Mass spectrometry 

摘      要：As the roles of glycans in health and disease continue to be unraveled,it is becoming apparent that glycans’immense complexity cannot be *** fully delineate glycan structures,we developed an integrative approach combining a set of cost-effective,widespread,and easy-to-handle analytical *** key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate(Fmoc)—to bridge the gap between diverse glycoanalytical methods,especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection(xCGELIF)and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOFMS).Through the detailed structural analysis of selected,dauntingly complex N-glycans from chicken ovalbumin,horse serum,and bovine transferrin,we illustrate the capabilities of the presented ***,this approach“visualizesN-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures,potentially providing useful new targets for biomarker discovery.