Synergistic utilization of carbon sources for efficient biosynthesis of N-acetylglucosamine

作     者：Yanni Pei Yuhan Wang Xiaolin Shen Jia Wang Xinxiao Sun Qipeng Yuan Yanni Pei;Yuhan Wang;Xiaolin Shen;Jia Wang;Xinxiao Sun;Qipeng Yuan

作者机构：State Key Laboratory of Chemical Resource EngineeringBeijing University of Chemical TechnologyBeijing100029China 

出 版 物：《Green Chemical Engineering》 (绿色化学工程（英文）)

年 卷 期：2023年第4卷第2期

页      面：233-238页

核心收录：

学科分类：0710[理学-生物学] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：supported by the National Key Research and Development Program of China(2018YFA0901800) the National Natural Science Foundation of China(21978015). 

主　　题：N-Acetylglucosamine Synergistic carbon utilization Glucose facilitated diffusion system Acetate 

摘      要：N-acetylglucosamine(GlcNAc)is an amino monosaccharide that has a variety of bioactivities and is widely used in pharmaceutical and food industries.Production of GlcNAc by chitin hydrolysis is limited by the supply of raw materials and encounters the risk of shellfish protein contamination.For efficient biosynthesis of GlcNAc,one challenge is to balance the carbon distribution between growth and production.Here,we applied the strategy of synergistic carbon utilization,in which glycerol supports cell growth and provides the acetyl group of GlcNAc while glucose serves as the precursor to glucosamine.The efficiency of GlcNAc production was stepwise improved by blocking the product re-uptake and degradation,strengthening the biosynthetic pathway and synergistically utilizing two carbon sources.With these efforts,the final strain produced 41.5 g/L GlcNAc with a yield of 0.49 g/g of total carbon sources.In addition,we also explored the feasibility of using acetate as a cheap carbon source to partly replace glycerol.This study provides a promising alternative strategy for sustainable and efficient pro-duction of GlcNAc.