Modernising autism spectrum disorder model engineering and treatment via CRISPR-Cas9:A gene reprogramming approach

作     者：Arushi Sandhu Anil Kumar Kajal Rawat Vipasha Gautam Antika Sharma Lekha Saha 

作者机构：Department of PharmacologyPost Graduate Institute of Medical Education and Research(PGIMER)Chandigarh 0172ChandigarhIndia 

出 版 物：《World Journal of Clinical Cases》 (世界临床病例杂志)

年 卷 期：2023年第11卷第14期

页      面：3114-3127页

核心收录：

学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学] 

主　　题：Autism spectrum disorder CRISPR-Cas9 Cellular models Organoids Animal models Therapeutic strategies 

摘      要：A neurological abnormality called autism spectrum disorder(ASD)affects how a person perceives and interacts with others,leading to social interaction and communication *** and recurring behavioural patterns are another feature of the *** mutations throughout development are the source of the neurodevelopmental disorder ***,a well-established model and perfect treatment for this spectrum disease has not been *** rising era of the clustered regularly interspaced palindromic repeats(CRISPR)-associated protein 9(Cas9)system can streamline the complexity underlying the pathogenesis of *** CRISPR-Cas9 system is a powerful genetic engineering tool used to edit the genome at the targeted site in a precise *** major hurdle in studying ASD is the lack of appropriate animal models presenting the complex symptoms of ***,CRISPR-Cas9 is being used worldwide to mimic the ASD-like pathology in various systems like in vitro cell lines,in vitro 3D organoid models and in vivo animal *** from being used in establishing ASD models,CRISPR-Cas9 can also be used to treat the complexities of *** aim of this review was to summarize and critically analyse the CRISPRCas9-mediated discoveries in the field of ASD.