Characterization of sulfide oxidation and optimization of sulfate production by a thermophilic Paenibacillus naphthalenovorans LYH-3 isolated from sewage sludge composting

作     者：Li Chen Weiguang Li Yi Zhao Shumei Zhang Liqiang Meng Yujie Zhou Li Chen;Weiguang Li;Yi Zhao;Shumei Zhang;Liqiang Meng;Yujie Zhou

作者机构：School of EnvironmentHarbin Institute of TechnologyHarbin 150090China State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbin 150090China Institute of MicrobiologyHeilongjiang Academy of SciencesHarbin 150010China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第125卷第3期

页      面：712-722页

核心收录：

学科分类：0710[理学-生物学] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 0703[理学-化学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(No. 51878216)。 

主　　题：Sulfide oxidizing bacteria Paenibacillus naphthalenovorans Thermophilic strains Optimization Growth kinetics Bacterial isolation 

摘      要：The sulfur-containing odor emitted from sludge composting could be controlled by sulfide oxidizing bacteria, yet mesophilic strains show inactivation during the thermophilic stage of composting. Aimed to investigate and characterize the thermotolerant bacterium that could oxidize sulfide into sulfate, a heterotrophic strain was isolated from sewage sludge composting and identified as Paenibacillus naphthalenovorans LYH-3. The effects of various environmental factors on sulfide oxidation capacities were studied to optimize the sulfate production, and the highest production rate (27.35%±0.86%) was obtained at pH 7.34, the rotation speed of 161.14 r/min, and the inoculation amount of 5.83%by employing BoxBehnken design. The results of serial sulfide substrates experiments indicated that strain LYH-3 could survive up to 400 mg/L of sulfide with the highest sulfide removal rate (88.79%±0.35%) obtained at 50 mg/L of sulfide. Growth kinetic analysis presented the maximum specific growth rateμm(0.5274 hr-1) after 22 hr cultivation at 50℃. The highest enzyme activities of sulfide quinone oxidoreductase (0.369±0.052 U/mg) and sulfur dioxygenase (0.255±0.014 U/mg) were both obtained at 40℃, and the highest enzyme activity of sulfite acceptor oxidoreductase (1.302±0.035 U/mg) was assessed at 50℃. The results indicated that P. naphthalenovorans possessed a rapid growth rate and efficient sulfide oxidation capacities under thermophilic conditions, promising a potential application in controlling sulfur-containing odors during the thermophilic stage of sludge composting.