Vitamin C induces periodontal ligament progenitor cell differentiation via activation of ERK pathway mediated by PELP1

作     者：Yan Yan Wenfeng Zeng Shujun Song Fayun Zhang Wenxi He Wei Liang Zhongying Niu 

作者机构：Department of EndodonticsSchool of StomatologyFourth Military Medical UniversityXi’an710032China Protein and Peptide Pharmaceutical LaboratoryInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Treatment Center of Oral Diseasesthe 306th Hospital of People’s Liberation ArmyBeijing 100101China Department of Pathology and Experimental Medicinethe 306th Hospital of People’s Liberation ArmyBeijing 100101China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2013年第4卷第8期

页      面：620-627页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 0703[理学-化学] 0836[工学-生物工程] 10[医学] 

基　　金：This work is supported by grants:the National Basic Research Program(973 Program)(No.2011CB707705) National Natural Science Foundation of China(Grant No.11202229). 

主　　题：periodontal ligament progenitor cells Vitamin C PELP1 ERK signaling pathway osteogenic differentiation 

摘      要：The differentiation of periodontal ligament(PDL)progenitor cells is important for maintaining the homeostasis of PDL tissue and alveolar bone.Vitamin C(VC),a water-soluble nutrient that cannot be biosynthesized by humans,is vital for mesenchymal stem cells differentiation and plays an important role in bone remodeling.Therefore,the objective of this study was to determine the function and mechanism of VC in PDL progenitor cells osteogenic differentiation at the molecular level.We demonstrated that VC could induce the osteogenic differentiation and maturation of PDL progenitor cell without other osteogenic agents.During the process,VC preferentially activated ERK1/2 but did not affect JNK or p38.Co-treatment with ERK inhibitor effectively decreased the Vitamin C-induced expression of Runx2.ERK inhibitor also abrogated Vitamin C-induced the minimized nodules formation.PELP1,a nuclear receptor co-regulator,was up-regulated under VC treatment.PELP1 knockdown inhibited ERK phosphorylation.The overexpression of PELP1 had a positive relationship with Runx2 expression.Taken together,we could make a conclude that VC induces the osteogenic differentiation of PDL progenitor cells via PELP1-ERK axis.Our fi nding implies that VC may have a potential in the regeneration medicine and application to periodontitis treatment.