Systematic identification of CRISPR off-target effects by CROss-seq

作     者：Yan Li Shengyao Zhi Tong Wu Hong-Xuan Chen Rui Kang Dong-Zhao Mai Zhou Songyang Chuan He Puping Liang Guan-Zheng Luo Yan Li;Shengyao Zhi;Tong Wu;Hong-Xuan Chen;Rui Kang;Dong-Zhao Ma;Zhou Songyang;Chuan He;Puping Liang;Guan-Zheng Luo

作者机构：IMOE Key Laboratory of Gene Function and RegulationGuangdong Province Key Laboratory of Pharmaceutical Functional GenesState Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Department of ChemistryUniversity of ChicagoChicagoIL 60637USA Institute for Biophysical DynamicsDepartment of Biochemistry and Molecular BiologyHoward Hughes Medical InstituteUniversity of ChicagoChicagoIL 60637 USA 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2023年第14卷第4期

页      面：299-303页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the Ministry of Science and Technology of China to G.Z.L.(National Science and Technology Major Project,grant nos.2018YFA0109100,2019YFA0802203) National Natural Science Foundation of China to G.z.L.(Grant Nos.31922015,31870808,91753129) Natural Science Foundation of Guangdong Province to G.Z.L.(Grant No.2018B030306044) Guangdong Special Support Program to P.L.(2019BT02Y276). 

主　　题：CRISPR versatile editor 

摘      要：DearEditor,The CRISPR-mediated genome editing tools,including nucleases,base editors(ABE/CBE),transposases/recombinases,and prime editor(PE),have been extensively applied in basic and clinical researches,although the off-target effect remains a major concern(Anzalone et al.,2020).Recently,various methods have been developed to assess the specificity and accuracy of different tools(Zhang et al.,2021),yet each method is designed for limited editing systems,and none of them can simultaneously detect off-target sites in vivo and in vitro.A versatile method for profiling genome-wide off-target effects of various tools remains lacking.