Schistosoma japonicum: Isolation and Identification of Peptides Mimicking Ferritin Epitopes from Phage Display Library

作     者：Lian-Fei TANG, Xin-Yuan YI*, Xian-Fang ZENG, Lin-Qian WANG, and Shun-Ke ZHANG Department of Parasitology, Central South University Xiangya School of Medicine, Changsha 410078, China Lian-Fei TANG, Xin-Yuan YI*, Xian-Fang ZENG, Lin-Qian WANG, and Shun-Ke ZHANG Department of Parasitology, Central South University Xiangya School of Medicine, Changsha 410078, China

作者机构：Department of Parasitology Central South University Xiangya School of Medicine Changsha 410078 China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2004年第36卷第3期

页      面：206-210页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：This work was supported by the grant from WHO/TDR (980268) 

主　　题：phage displayed peptide library epitope Schistosoma japonicum ferritin 

摘      要：In an attempt to isolate and identify the antigenic epitopes on ferritin of Schistosoma japonicum (SjFer) and to test their protective potentiality against Schistosoma japonicum (S.j), polyclonal antisera against SjFer was prepared to screen a 12-mer random peptide library. Three rounds of biopan- ning were performed and resulted in an enrichment. Six peptides selected randomly from the third elute were all found to be positive by evaluating the binding to anti-SjFer sera by ELISA and Western blot- ting. Three amino acid sequences were deduced from the six phage clones by sequencing. When they were used to vaccinate mice, the three peptides could induce significant reduction in adult worms (26.7%, 20.4%, and 25.9%) as well as in liver eggs per gram (LEPG) (40.0%, 38.2%, and 40.8%). This result showed that three mimotopes on SjFer were obtained and they could induce significant protective efficacy against S.j.