Multiple endocrine factors regulate nutrient mobilization and storage in Aedes aegypti during a gonadotrophic cycle

作     者：Xiaoyi Dou Kangkang Chen Mark R.Brown Michael R.Strand Xiaoyi Dou;Kangkang Chen;Mark R.Brown;Michael R.Strand

作者机构：Department of EntomologyUniversity of GeorgiaAthensGeorgia30602United States Department of Plant ProtectionCollege of Horticulture and Plant ProtectionYangzhou UniversityYangzhouJiangsu ProvinceChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2023年第30卷第2期

页      面：425-442页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 

基　　金：provided by the National Institutes of Health(R01AI033108,R01AI106892)awarded to MRS and MRB National Science Foundation(IOS 1656236)awarded to MRS and MRB United States Department of Agriculture Hatch Project GEO00772 to MRS the Pulliam Endowment(MRS) partially supported by the National Natural Science Foundation(31901876) the Natural Science Foundation of Jiangsu Province(KB20190900). 

主　　题：adipokinetic hormone ecdysone endocrine glycogen insulin-like peptide juvenile hormone mosquito nutrient ovary ecdysteroidogenic hormone triacylglycerol 

摘      要：Anautogenous mosquitoes must blood feed on a vertebrate host to produce eggs.Each gonadotrophic cycle is subdivided into a sugar-feeding previtellogenic phase that produces primary follicles and a blood meal-activated vitellogenic phase in which large numbers of eggs synchronously mature and are laid.Multiple endocrine factors including juvenile hormone(JH),insulin-like peptides(ILPs),ovary ecdysteroidogenic hormone(OEH),and 20-hydroxyecdysone(20E)coordinate each gonadotrophic cycle.Egg formation also requires nutrients from feeding that are stored in the fat body.Regulation of egg formation is best understood in Aedes aegypti but the role different endocrine factors play in regulating nutrient mobilization and storage remains unclear.In this study,we report that adult female Ae.aegypti maintained triacylglycerol(TAG)stores during the previtellogenic phase of the first gonadotrophic cycle while glycogen stores declined.In contrast,TAG and glycogen stores were rapidly mobilized during the vitellogenic phase and then replenishment.Several genes encoding enzymes with functions in TAG and glycogen metabolism were differentially expressed in the fat body,which suggested regulation was mediated in part at the transcriptional level.Gain of function assays indicated that stored nutrients were primarily mobilized by adipokinetic hormone(AKH)while juvenoids and OEH regulated replenishment.ILP3 further showed evidence of negatively regulating certain lipolytic enzymes.Loss of function assays indicated AKH depends on the AKH receptor(AKHR)for function.Altogether,our results indicate that the opposing activities of different hormones regulate nutrient stores during a gonadotrophic cycle in Ae.aegypti.