Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues

作     者：Arwa Daghrery Jessica A.Ferreira Jinping Xu Nasim Golafshan Darnell Kaigler Sarit B.Bhaduri Jos Malda Miguel Castilho Marco C.Bottino 

作者机构：Department of CariologyRestorative Sciencesand EndodonticsSchool of DentistryUniversity of MichiganAnn ArborMIUnited States Department of Restorative Dental SciencesSchool of DentistryJazan UniversityJazanKingdom of Saudi Arabia Department of OrthopedicsUniversity Medical Center UtrechtUtrechtthe Netherlands Regenerative Medicine CenterUtrechtthe Netherlands Department of Periodontics and Oral MedicineSchool of DentistryUniversity of MichiganAnn ArborMIUnited States Department of MechanicalIndustrial and Manufacturing EngineeringUniversity of ToledoToledoOHUnited States EEC DivisionDirectorate of EngineeringThe National Science FoundationAlexandriaVAUnited States Department of Clinical SciencesFaculty of Veterinary MedicineUtrecht UniversityUtrechtthe Netherlands Department of Biomedical EngineeringEindhoven University of TechnologyEindhoventhe Netherlands Institute for Complex Molecular SystemsEindhoven University of TechnologyP.O.Box 5135600 MB Eindhoventhe Netherlands Department of Biomedical EngineeringCollege of EngineeringUniversity of MichiganAnn ArborMIUnited States 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第19卷第1期

页      面：268-281页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：the National Institutes of Health(NIH-National Institute of Dental and Craniofacial Research grants K08DE023552 R01DE026578) 

主　　题：Periodontitis Melt electrowriting 3D printing Inflammation Periodontal regeneration Scaffold 

摘      要：Periodontitis is a chronic inflammatory condition that often causes serious damage to tooth-supporting *** limited successful outcomes of clinically available approaches underscore the need for therapeutics that cannot only provide structural guidance to cells but can also modulate the local immune ***,three-dimensional melt electrowritten(i.e.,poly(ε-caprolactone))scaffolds with tissue-specific attributes were engineered to guide differentiation of human-derived periodontal ligament stem cells(hPDLSCs)and mediate macrophage *** investigated tissue-specific scaffold attributes comprised fiber morphology(aligned ***)and highly-ordered architectures with distinct strand spacings(small 250μm and large 500μm).Macrophages exhibited an elongated morphology in aligned and highly-ordered scaffolds,while maintaining their round-shape on randomly-oriented fibrous *** of periostin and IL-10 were more pronounced on the aligned and highly-ordered *** hPDLSCs on the scaffolds with 500μm strand spacing show higher expression of osteogenic marker(Runx2)over 21 days,cells on randomly-oriented fibrous scaffolds showed upregulation of M1 *** an orthotopic mandibular fenestration defect model,findings revealed that the tissue-specific scaffolds(i.e.,aligned fibers for periodontal ligament and highly-ordered 500μm strand spacing fluorinated calcium phosphate[F/CaP]-coated fibers for bone)could enhance the mimicking of regeneration of natural periodontal tissues.