Cobalt protoporphyrin-induced nano-self-assembly for CT imaging,magnetic-guidance,and antioxidative protection of stem cells in pulmonary fibrosis treatment

作     者：Yimeng Shu Ming Ma Xiaoxia Pan Muhammad Shafiq Huizhu Yu Hangrong Chen 

作者机构：State Key Laboratory of High Performance Ceramics and Superfine Microstructures Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050PR China Center of Materials Science and Optoelectronics EngineeringUniversity of Chinese Academy of SciencesBeijing100049PR China School of Chemistry and Materials ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences1 Sub-laneXiangshanHangzhou310024PR China State Key Laboratory of Molecular Engineering of PolymersDepartment of Macromolecular ScienceFudan UniversityShanghai200438PR China Department of BiotechnologyFaculty of Science and TechnologyUniversity of Central Punjab(UCP)Lahore54000Pakistan 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第21卷第3期

页      面：129-141页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：National Natural Science Foundation of China(Grant Nos.52072392,32030061,82171951) Key Program for Basic Research of Shanghai(Grant Nos.20JC1411900,21JC1406000) Shanghai Rising-Star Program(Grant No.19QA1410300) Youth Innovation Promotion Association CAS(Grant No.2020255). 

主　　题：Self-assembly Pulmonary fibrosis Cell labeling Stem cell therapy Theranostic 

摘      要：Mesenchymal stem cells(MSCs)transplantation is a promising approach for pulmonary fibrosis(PF),however it is impeded by several persistent challenges,including the lack of long-term tracking,low retention,and poor survival of MSCs,as well as the low labeling efficiency of nanoprobes.Herein,a cobalt protoporphyrin IX(CoPP)aggregation-induced strategy is applied to develop a multifunctional nano-self-assembly(ASCP)by combining gold nanoparticle(AuNPs),superparamagnetic iron oxide nanoparticles(SPIONs),and CoPP through a facile solvent evaporation-driven approach.Since no additional carrier materials are employed during the synthesis,high loading efficiency of active ingredients and excellent biocompatibility are achieved.Additionally,facile modification of the ASCPs with bicyclo[6.1.0]nonyne(BCN)groups(named as ASCP-BCN)enables them to effectively label MSCs through bioorthogonal chemistry.The obtained ASCP-BCN could not only help to track MSCs with AuNP-based computed tomography(CT)imaging,but also achieve an SPIONs-assisted magnetic field based improvement in the MSCs retention in lungs as well as promoted the survival of MSCs via the sustained release of CoPP.The in vivo results demonstrated that the labeled MSCs improved the lung functions and alle-viated the fibrosis symptoms in a bleomycin–induced PF mouse model.Collectively,a novel ASCP-BCN multi-functional nanoagent was developed to bioorthogonally-label MSCs with a high efficiency,presenting a promising potential in the high-efficient MSC therapy for PF.