Bioprinted constructs that simulate nerve-bone crosstalk to improve microenvironment for bone repair

作     者：Tianchang Wang Wentao Li Yuxin Zhang Xiang Xu Lei Qiang Weiqiang Miao Xiaokun Yue Xin Jiao Xianhao Zhou Zhenjiang Ma Shuai Li Muliang Ding Junfeng Zhu Chi Yang Hui Wang Tao Li Xin Sun Jinwu Wang 

作者机构：Shanghai Key Laboratory of Orthopedic ImplantDepartment of Orthopedic SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineNo.639 Zhizaoju RoadShanghai200011China Sports Medicine DepartmentBeijing Key Laboratory of Sports InjuriesPeking University Third HospitalNo.49North Garden RoadHaidian DistrictBeijing100191China Peking University Institute of Sports MedicineNo.49North Garden RoadHaidian DistrictBeijing100191China Department of Oral SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghai200011China School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu610031China Department of OrthopedicsThe First Affiliated HospitalZhejiang University School of Medicine79 Qingchun RdHangzhou310003China Department of OrthopaedicsThe Second Xiangya HospitalCentral South UniversityChangsha410001HunanChina Institute of Rehabilitation MedicineSchool of Rehabilitation ScienceShanghai University of Traditional Chinese MedicineEngineering Research Center of Traditional Chinese Medicine Intelligent RehabilitationMinistry of EducationShanghai201210China Department of Orthopedic SurgeryXin Hua Hospital Affiliated with Shanghai Jiao Tong University School of MedicineNo.1665Kongjiang RoadShanghai200092China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第27卷第9期

页      面：377-393页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：support from National Key R&D Program of China(2018YFA0703000) National Natural Science Foundation of China(82072412) Translation Medicine National Key Science and Technology Infrastructure(Shanghai)Open Project(TMSK-2020-118) Lingang Laboratory“Seeking Outstanding Youth Program”open project(LG-QS-202206-04) 

主　　题：Schwann cells Microenvironment Nerve-bone crosstalk Exosomes Bioprinting 

摘      要：Crosstalk between nerves and bone is essential for bone repair,for which Schwann cells(SCs)are crucial in the regulation of the *** that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair,the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes(SC-exos)on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve-bone *** promoted the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs)through *** molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-βsignaling pathway via ***,SC-exos promoted the migration and tube formation performance of endothelial progenitor ***,the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve-bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos,exerting the regulatory effect of SCs in the microenvironment to promote innervation,vascularization,and osteogenesis and thus effectively improving bone repair in a cranial defect *** study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair.