Gelatin-based hydrogel functionalized with taurinemoieties for in vivo skin tissue regeneration

作     者：Farnaz Rahimi Nima Ahmadkhani Aida Goodarzi Fariba Noori Sajad Hassanzadeh Sepideh Saghati Mehdi Khanmohammadi Arash Goodarzi Farnaz Rahimi;Nima Ahmadkhani;Aida Goodarzi;Fariba Noori;Sajad Hassanzadeh;Sepideh Saghati;Mehdi Khanmohammadi;Arash Goodarzi

作者机构：Department of Tissue EngineeringSchool of Advanced Technologies in MedicineFasa University of Medical ScienceFasaIran School of ChemicalBiological and Environmental EngineeringOregon State UniversityCorvallisOR 97331USA Eye Research CenterSchool of MedicineThe Five Senses Health InstituteIran University of Medical SciencesTehranIran Skull Base Research CenterSchool of MedicineThe Five Senses Health InstituteIran University of Medical SciencesTehranIran Department of Tissue EngineeringFaculty of Advanced Medical SciencesTabriz University of Medical SciencesTabrizIran 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2023年第6卷第3期

页      面：284-297页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 081702[工学-化学工艺] 0817[工学-化学工程与技术] 08[工学] 0807[工学-动力工程及工程热物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0802[工学-机械工程] 0836[工学-生物工程] 0811[工学-控制科学与工程] 

主　　题：Taurine conjugation Covalent crosslinking Immobilization of growth factors Gelatin-based hydrogel Endothelial cells 

摘      要：Functionalized hydrogels stimulate the migration and morphogenesis of endothelial cells(ECs)and are useful substrates for wound *** present study investigates the feasibility of covalent conjugation of taurine(Tau)on a gelatin-based *** hydrogel is expected to maintain positive charged growth factors such as basic fibroblast growth factor(bFGF)and vascular endothelial growth factors(VEGFs)near ECs within the hydrogel *** gelatin was conjugated with hydroxyl phenol(Ph)and Tau moieties,and in following that Ph residues were crosslinked through a horseradish peroxidase-catalyzed *** migration characteristics of ECs were analyzed by scratch migration assay and microparticle-based cell migration *** morphology and amounts of angiopoietin 1(Ang 1),bFGF,and VEGF proteins were evaluated for encapsulated *** potential of synthesized hydrogels in wound healing was assessed by the percentage of reduction from the original wound size and histopathological analyses of rat *** incorporated Tau molecules within the hydrogel remained stable through covalent bonds during *** extended incubation,the gelatin-based hydrogel conjugated with Tau improved the migration distance and number of existing migrated *** Tau within the gelatin-based hydrogel induced high motility of ECs,accompanied by robust cytoskeleton extension and a cell subpopulation that expressed CD44 and CD31 receptors as well as enhancement of Ang 1,bFGF,and *** found that injectable Gel-Ph-Tau effectively improves wound-healing parameters.