Antibacterial activity and mechanism of newly developed Zr-30Ta and Zr-25Ta-5Ti alloys against implant-associated infection

作     者：Hai-Lin Yang Akram Nasser Juaim Ling Zou Meng-Zhen Zhu Xiao-Na Chen Chen-Xu Ma Xiong-Wen Zhou Hai-Lin Yang;Akram Nasser Juaim;Ling Zou;Meng-Zhen Zhu;Xiao-Na Chen;Chen-Xu Ma;Xiong-Wen Zhou

作者机构：State Key Laboratory of Powder MetallurgyCentral South UniversityChangsha 410083China National Clinical Research Center for Geriatric DisordersXiangya HospitalCentral South UniversityChangsha 410008China Department of ProsthodonticsCenter of StomatologyXiangya HospitalCentral South UniversityChangsha 410008China Department of ProsthodonticsFaculty of DentistryThamar UniversityDharmarYemen Department of Oral ImplantologyXiangya Stomatological Hospital and School of StomatologyCentral South UniversityChangsha 410008China 

出 版 物：《Rare Metals》 (稀有金属（英文版）)

年 卷 期：2022年第41卷第12期

页      面：4176-4187页

核心收录：

学科分类：08[工学] 0806[工学-冶金工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：financially supported by the Natural Science Foundation of Hunan Province(Nos.2020JJ4732 and 2020JJ4933) the National Natural Science Foundation of China(No.52071343) 

主　　题：Zirconium-tantalum Dental implant Antibacterial Peri-implant infection Biofilm 

摘      要：Infection is a common problem after implantation,and the currently used Ti-based implants have insufficient antibacterial activities.The newly developed Zr-30Ta and Zr-25Ta-5Ti alloys exhibited good mechanical properties,low elastic moduli,and high corrosion resistance.In this work,their antibacterial activities and antibacterial mechanisms were investigated.The surface roughness,wettability,and charge of the alloys were evaluated using a profilometer,drop shape analyzer,and electrokinetic analyzer,respectively.The antibacterial activity against Streptococcus mutans and Porphyromonas gingivalis was investigated using the inhibition zone assay,plate count assay,live/dead staining,and scanning electron microscopy(SEM).The reactive oxygen species release(ROS)was evaluated using the 2’7’-dichlorodihydrofluorescein diacetate(DCFH-DA)assay.The results demonstrated that Zr-30Ta and Zr-25Ta-5Ti alloys had favorable smooth,hydrophilic,negatively charged surfaces that decreased bacteria adhesion and prevented biofilm formation.Zr-30Ta and Zr-25Ta-5Ti alloys also exhibited a time-dependent decrease in the bacteria adhesion and viability.Zr-30Ta and Zr-25Ta-5Ti alloys were also found to induce direct membrane damage and increase ROS production,resulting in significantly better antibacterial activities than CP-Ti.Zr-30Ta and Zr-25Ta-5Ti alloys exhibited comparable antibacterial activities.This work suggests that the newly developed Zr-30Ta and Zr-25Ta-5Ti alloys have good antibacterial activity against Streptococcus mutans and Porphyromonas gingivalis and are promising candidate materials for dental and orthopedic implants.