Recent Advances in Single-Cell Metabolomics Based on Mass Spectrometry
作者机构：Department of Chemistry and Applied BiosciencesETH ZurichCH-8093 Zurich
出 版 物：《CCS Chemistry》 (中国化学会会刊（英文）)
年 卷 期：2023年第5卷第2期
页 面：310-324页
核心收录：
学科分类：07[理学] 070302[理学-分析化学] 0703[理学-化学]
基 金：the China Scholarship Council for financially supporting her Ph.D. research
主 题：single-cell analysis metabolomics mass spectrometry ionization techniques analytical methods
摘 要：Cellular heterogeneity is essential for the physiological functions of organisms,and precise interpretation of the relevant biological mechanisms involved requires accurate measurement of biomolecules,including DNA,RNA,proteins,and metabolites at the single-cell level.Cellular metabolites react most rapidly to environmental and biochemical changes such that their detection is a critical step in determining the physiological state of cells.However,their limited amounts,structural diversity,and significant content variation render single-cell metabolomics analysis technically challenging.Mass spectrometry,with its high sensitivity,good selectivity,and wide dynamic range,has been widely used in the single-cell analysis in recent years.In terms of ionization techniques,there are four main types of single-cell mass spectrometry methods:nano-electrospray ionization mass spectrometry,matrix-assisted laser desorption/ionization mass spectrometry,secondary ion mass spectrometry,and dielectric barrier discharge ionization mass spectrometry.In this mini review,the progress of single-cellmass spectrometrymethodologies and data processing strategies are summarized,and the future trends in single-cell mass spectrometry are outlined.
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