Characteristics and biological control functions of Bacillus sp.PM8313 as a host-associated probiotic in red sea bream(Pagrus major)aquaculture

作     者：Won Je Jang Kyung-Bon Lee Mi-Hyeon Jeon Su-Jeong Lee Sang Woo Hur Seunghan Lee Bong-Joo Lee Jong Min Lee Kang-Woong Kim Eun-Woo Lee Won Je Jang;Kyung-Bon Lee;Mi-Hyeon Jeon;Su-Jeong Lee;Sang Woo Hur;Seunghan Lee;Bong-Joo Lee;Jong Min Lee;Kang-Woong Kim;Eun-Woo Lee

作者机构：Department of BiotechnologyPukyong National UniversityBusan48513South Korea Department of Biology Education College of EducationChonnam National UniversityGwangju61186South Korea Biopharmaceutical Engineering MajorDivision of Applied BioengineeringDong-Eui UniversityBusan47340South Korea Aquafeed Research CenterNational Institute of Fisheries SciencePohang37517South Korea Department of Smart Fisheries ResourcesCollege of Industrial SciencesKongju National UniversityYesan32439South Korea 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第12卷第1期

页      面：20-31页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 

基　　金：financially supported by the grant (R2022016) from the National Institute of Fisheries Science,Republic of Korea the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2022R1A2C1012655). 

主　　题：Host-associated probiotics Immunity Microbiota Probiotics Red sea bream 

摘      要：Host-associated probiotics(HAPs)are bacteria originally isolated from rearing water or the host s gastrointestinal tract in order to enhance the host s growth and health.This study investigated the HAP potential of Bacillus sp.PM8313,isolated from wild red sea bream(Pagrus major),through characterization and feeding trials.Results based on in vitro tests showed that PM8313 is safe,confirming its hemolytic,cytotoxic,and antibiotic resistance.In addition,PM8313 showed advantages as a probiotic with high viability in the gastrointestinal model and a high cell adhesion rate.Whole-genome sequencing demonstrated that PM8313 has a 4,615,871 bp single circular chromosome and a guaninecytosine content of 45.25%.It also showed the absence of genes encoding virulence factors,such as cytotoxin,enterotoxin,hemolysin,sphingomyelinase,and phospholipase.In the feeding trial,a supplemental diet of 1×10^(8)CFU/g PM8313 positively altered the weight gain,digestive enzyme activity,and intestinal microbiota composition of red sea bream.Analysis of nonspecific immune parameters and immune-related gene expression,and a challenge test showed that PM8313 supplementation increases immunity and pathogenic bacteria resistance.Our findings suggest that PM8313 should be considered for application as a novel HAP to red sea bream aquaculture.