Reproductive toxicity of organic extracts from petrochemical plant effluents discharged to the Yangtze River,China

作     者：Xiaoyi Wang Wei Shi Jiang Wu Yingqun Hao Guanjiu Hu Hongling Liu Xiaodong Han Hongxia Yu 

作者机构：The State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China. Immunology and Reproductive Biology Laboratory Medical School Nanjing University Nanjing 210093 China State Environmental Protection Key Laboratory of Monitoring and Analysis for Organic Pollutants in Surface Water Jiangsu Provincial Environmental Monitoring Center Nanjing 210036 China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2010年第22卷第2期

页      面：297-303页

核心收录：

学科分类：0905[农学-畜牧学] 08[工学] 09[农学] 0815[工学-水利工程] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by the Major State Basic Research Development Program (No.2008CB418102) the Environmental Monitoring Research Foundation of Jiangsu Province (No.0710) the Innovation Foundation for Youth Scholars of the State Key Laboratory of Pollution Control and Resource Reuse (No.PCRREF07002) the Specialized Research Fund for the Doctoral Program of Higher Education, SRFDP (No.200802841030) 

主　　题：wastewater in vitro system sertoli cells spermatogenic ceils leydig cells 

摘      要：Water pollution of the Yangtze River in China became one of challenges that the government is facing today. Increasing numbers of petrochemical plants were built along the river in past decades, and numbers of organic chemicals were discharged into the river. Our goal was to establish in vitro system on rat sertoli cells, spermatogenic cells and leydig cells to investigate the reproductive toxicity potential induced by organic extracts from petrochemical effluents. Our results showed that the organic extract depressed the viability （p 〈 0.01） and destroyed the plasma membrane integrity of sertoli cells and spermatogenic cells to a certain degree. Accordingly, proportion of early apoptotic sertoli cells and late apoptotic spermatogenic cells increased significantly. Although significant morphological changes were not detected for leydig cells, the extract was observed to inhibit their testosterone production （p 〈 0.01）. Sertoli cells and sperrnatogenic cells appeared to be more sensitive and maybe the main targets of the key toxins. These results suggested that the in vitro system on rat testicular cells may be useful to predicate reproductive toxicity potential of organic extracts from petrochemical effluents. More attention should be paid to the petrochemical effluents, because long-term accumulation of these organic compounds in organisms may cause spermatogenesis malfunction and testosterone reduction.