m^(6)A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte

作     者：Kan Li Weichen Huang Zhijun Wang Qinghua Nie Kan Li;Weichen Huang;Zhijun Wang;Qinghua Nie

作者机构：Department of Animal GeneticsBreeding and ReproductionCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China National‑Local Joint Engineering Research Center for Livestock BreedingGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breedingand Key Laboratory of Chicken GeneticsBreeding and ReproductionMinistry of AgricultureGuangzhou 510642China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2023年第14卷第2期

页      面：578-592页

核心收录：

学科分类：090601[农学-基础兽医学] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 

基　　金：funded by the Natural Scientific Foundation of China(U1901206) Local Innovative and Research Teams Project of Guangdong Province(2019BT02N630) the Science and Technology Program of Guangdong province,China(2020B1212060060) the Science and Technology Program of Guangzhou,China(202103000084) the Construction Project of Modern Agricultural Science and Technology Innovation Alliance in Guangdong Province(2021KJ128) National Key R&D Program of China(2021YFD1300100) China Agriculture Research System(CARS-41-G03) 

主　　题：Adipogenesis Chicken CTNNB1 FTO m^(6)A 

摘      要：Background:N6-methyladenosine(m^(6)A)is an abundant post-transcriptional RNA modification that affects various biological *** fat mass and obesity-associated(FTO)protein,a demethylase encoded by the FTO gene,has been found to regulate adipocyte development in an m^(6)A-dependent manner in multiple ***,the effects of the m^(6)A methylation and FTO demethylation functions on chicken adipogenesis remain *** study aims to explore the association between m^(6)A modification and chicken adipogenesis and the underlying mechanism by which FTO affects chicken preadipocyte ***:The association between m^(6)A modification and chicken lipogenesis was assessed by treating chicken pread-ipocytes with different doses of methyl donor betaine and methylation inhibitor *** results showed that betaine significantly increased methylation levels and inhibited lipogenesis,and the inverse effect was found in preadipocytes after cycloleucine *** of FTO significantly inhibited m^(6)A levels and promoted proliferation and differentiation of chicken *** FTO showed opposite ***,FTO overexpression increased the expression of catenin beta 1(CTNNB1)by improving RNA stability in an m^(6)A-dependent manner,and we proved that FTO could directly target ***,CTNNB1 may be a positive regulator of adipogenesis in chicken ***:m^(6)A methylation of RNA was negatively associated with adipogenesis of chicken *** could regulate CTNNB1 expression in a demethylation manner to promote lipogenesis.