Development of an efficient expression system with large cargo capacity for interrogation of gene function in bamboo based on bamboo mosaic virus

作     者：Yandong Jin Baijie Wang Mingchuan Bao Yujie Li Shengwu Xiao Yuhua Wang Jun Zhang Liangzhen Zhao Hangxiao Zhang Yau-Heiu Hsu Mingjie Li Lianfeng Gu Yandong Jin;Baijie Wang;Mingchuan Bao;Yujie Li;Shengwu Xiao;Yuhua Wang;Jun Zhang;Liangzhen Zhao;Hangxiao Zhang;Yau-Heiu Hsu;Mingjie Li;Lianfeng Gu

作者机构：College of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China Basic Forestry and Proteomics Research CenterSchool of Future TechnologyCollege of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China Graduate Institute of BiotechnologyChung Hsing UniversityTaichung 40227China College of crop scienceFujian Agriculture and Forestry UniversityFuzhou 350002China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第6期

页      面：1369-1382页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：funded by the National Key Research and Development Program of China(2021YFD2200505) a grant from the National Natural Science Foundation of China(31971734) the Natural Science Foundation of Fujian Province(2021J02027) the Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University(72202200205) 

主　　题：ACE1 bamboo BaMV-mediated overexpression DEC1 expression virus-mediated genome editing 

摘      要：Bamboo is one of the fastest growing plants among monocotyledonous species and is grown extensively in subtropical *** bamboo has high economic value and produces much biomass quickly,gene functional research is hindered by the low efficiency of genetic transformation in this *** therefore explored the potential of a bamboo mosaic virus(BaMV)-mediated expression system to investigate genotype-phenotype *** determined that the sites between the triple gene block proteins(TGBps)and the coat protein(CP)of BaMV are the most efficient insertion sites for the expression of exogenous genes in both monopodial and sympodial bamboo ***,we validated this system by individually overexpressing the two endogenous genes ACE1 and DEC1,which resulted in the promotion and suppression of intemode elongation,*** particular,this system was able to drive the expression of three 2A-linked betalain biosynthesis genes(more than 4 kb in length)to produce betalain,indicating that it has high cargo capacity and may provide the prerequisite basis for the development of a DNA-free bamboo genome editing platform in the *** BaMV can infect multiple bamboo species,we anticipate that the system described in this study will greatly contribute to gene function research and further promote the molecular breeding of bamboo.