Engineered high-strength biohydrogel as a multifunctional platform to deliver nucleic acid for ameliorating intervertebral disc degeneration

作     者：Tao Chen Qiuping Qian Pooyan Makvandi Ehsan Nazarzadeh Zare Qizhu Chen Linjie Chen Zhiguang Zhang Hao Zhou Wenxian Zhou Hui Wang Xiangyang Wang Yu Chen Yunlong Zhou Aimin Wu 

作者机构：Department of OrthopaedicsKey Laboratory of Orthopaedics of Zhejiang ProvinceThe Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang325000China Zhejiang Engineering Research Center for Tissue Repair MaterialsWenzhou InstituteUniversity of Chinese Academy of SciencesWenzhouZhejiang325000China Institute for BioengineeringSchool of EngineeringThe University of EdinburghEdinburghEH93FBUK School of ChemistryDamghan UniversityDamghan36716-45667Iran Wenzhou Medical UniversityWenzhouZhejiang325000China Materdicine LabSchool of Life SciencesShanghai UniversityShanghai200444China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第25卷第7期

页      面：107-121页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 10[医学] 

基　　金：This work was supported by Zhejiang Provincial Natural Science Foundation of China(LGF21H060010) National Natural Science Foundation of China(82272555) Zhejiang Provincial Traditional Chinese Medicine Science and Technology Program(2020ZB146) Zhejiang Provincial Medical Technology Foundation of China(2022PY071) Wenzhou Major Scientific and Technological Innovation Project(ZY2022010) Wenzhou Public Welfare Science and Technology Project(WIUCASQD2019001) 

主　　题：Intervertebral disc degeneration Hydrogel Nucleic acid MicroRNA Delivery Extracellular matrix 

摘      要：Intervertebral disc degeneration(IVDD)is a leading cause of low back *** strategy of using functional materials to deliver nucleic acids provides a powerful tool for ameliorating ***,the immunogenicity of nucleic acid vectors and the poor mechanical properties of functional materials greatly limit their ***,antagomir-204-3p(AM)shows low immunogenicity and effectively inhibits the apoptosis of nucleus pulposus ***,a high-strength biohydrogel based on zinc-oxidized sodium alginate-gelatin(ZOG)is designed as a multifunctional nucleic acid delivery *** loaded with AM(ZOGA)exhibits great hygroscopicity,antibacterial activity,biocompatibility,and ***,ZOGA can be cross-linked with nucleus pulposus tissue to form a high-strength collagen network that improves the mechanical properties of the intervertebral disc(IVD).In addition,ZOGA provides an advantageous microenvironment for genetic expression in which AM can play an efficient role in maintaining the metabolic balance of the extracellular *** results of the radiological and histological analyses demonstrate that ZOGA restores the height of the IVD,retains moisture in the IVD,and maintains the tissue *** ZOGA platform shows the sustained release of nucleic acids and has the potential for application to ameliorate IVDD,opening a path for future studies related to IVD.