Adhesive cryogel particles for bridging confined and irregular tissue defects

作     者：Yao-Ting Xue Ming-Yu Chen Jia-Sheng Cao Lei Wang Jia-Hao Hu Si-Yang Li Ji-Liang Shen Xin-Ge Li Kai-Hang Zhang Shu-Qiang Hao Sarun Juengpanich Si-Bo Cheng Tuck-Whye Wong Xu-Xu Yang Tie-Feng Li Xiu-Jun Cai Wei Yang Yao-Ting Xue;Ming-Yu Chen;Jia-Sheng Cao;Lei Wang;Jia-Hao Hu;Si-Yang Li;Ji-Liang Shen;Xin-Ge Li;Kai-Hang Zhang;Shu-Qiang Hao;Sarun Juengpanich;Si-Bo Cheng;Tuck-Whye Wong;Xu-Xu Yang;Tie-Feng Li;Xiu-Jun Cai;Wei Yang

作者机构：Department of Engineering MechanicsZhejiang UniversityHang‑zhou 310027China Key Laboratory of Soft Machines and Smart Devices of Zhejiang ProvinceZhejiang UniversityHangzhou 310027China Center for X‑MechanicsDepartment of Engineering MechanicsZhejiang Univer‑sityHangzhou 310027China Department of General SurgerySir Run-Run Shaw HospitalZhejiang UniversityHangzhou 310016China Soft Intelligent Materials Co.LtdSuzhou 215123China School of Biomedical Engineering and Health Sciences and Advanced Membrane Technology Research CentreUniversiti Teknologi Malaysia81310 SkudaiMalaysia 

出 版 物：《Military Medical Research》 (军事医学研究（英文版）)

年 卷 期：2023年第10卷第6期

页      面：763-777页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：supported by the National Natural Science Foundation of China (12102388, T2125009, 92048302) the National Key Research and Development Program of China 2017 (YFA0701100) the Fundamental Research Funds for the Central Universities (226-2022-00141, 2022QZJH52) 

主　　题：Tissue reconstruction Wet adhesion Adhesive hydrogel Bioadhesive 

摘      要：Background Reconstruction of damaged tissues requires both surface hemostasis and tissue *** with damage resulting from physical trauma or surgical treatments may have arbitrary surface topographies,making tissue bridging *** This study proposes a tissue adhesive in the form of adhesive cryogel particles(ACPs) made from chitosan,acrylic acid,1-ethyl-3-(3-dimethylaminopropyl) carbodiimide(EDC) and N-hydroxysuccinimide(NHS).The adhesion performance was examined by the 180-degree peel test to a collection of tissues including porcine heart,intestine,liver,muscle,and *** of ACPs was evaluated by cell proliferation of human normal liver cells(LO2)and human intestinal epithelial cells(Caco-2).The degree of inflammation and biodegradability were examined in dorsal subcutaneous rat *** ability of ACPs to bridge irregular tissue defects was assessed using porcine heart,liver,and kidney as the ex vivo ***,a model of repairing liver rupture in rats and an intestinal anastomosis in rabbits were established to verify the effectiveness,biocompatibility,and applicability in clinical *** ACPs are applicable to confined and irregular tissue defects,such as deep herringbone grooves in the parenchyma organs and annular sections in the cavernous *** formed tough adhesion between tissues[(670.9±50.1) J/m^(2) for the heart,(607.6±30.0) J/m^(2) for the intestine,(473.7±37.0) J/m^(2) for the liver,(186.1±13.3) J/m^(2) for the muscle,and(579.3±32.3) J/m^(2) for the stomach].ACPs showed considerable cytocompatibility in vitro study,with a high level of cell viability for 3 d[(98.8±1.2)%for LO2 and(98.3±1.6)%for Caco-2].It has comparable inflammation repair in a ruptured rat liver(P=0.58 compared with suture closure),the same with intestinal anastomosis in rabbits(P=0.40 compared with suture anastomosis).Additionally,ACP-based intestinal anastomosis(less than 30 s) was remarkably faster than the convention