RECENT PROGRESS IN MULTIFOCAL MULTIPHOTON MICROSCOPY

作     者：JUNLE QU LIXIN LIU YONGHONG SHAO HANBEN NIU BRUCE Z.GAO 

作者机构：College of Optoelectronic EngineeringShenzhen University Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceShenzhen 518060P.R.China School of Technical PhysicsXidian University Xi'an 710071P.R.China Department of Bioengineering and COMSET Clemson UniversityClemson SC 29634USA 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2012年第5卷第3期

页      面：41-48页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 070207[理学-光学] 0808[工学-电气工程] 07[理学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：This work has been partially supported by NIH(SC COBRE P20RR021949 and Career Award 1k25hl088262-01) NSF(MRI CBET-0923311 and SC EPSCoR RII EPS-0903795 through SC GEAR program) The National Natural Science Foundation of China(31171372,61078067) Guangdong Province Science and Technology Project(2010B060300002) Shenzhen University Application Technology Development Project(201136,CXB201104220021A,JC201005250032A,200854) the Fundamental Research Funds for the Central Universities(K50510050006). 

主　　题：Multifocal multiphoton microscopy(MMM) microlens array beamsplitter diffractive optical element(DOE) spatial light modulator(SLM) 

摘      要：Multifocal multiphoton microscopy(MMM)has recently become an important tool in biomedicine for performing three-dimensional fastfluorescence imaging.Using various beamsplitting techniques,MMM splits the near-infrared laser beam into multiple beamlets and produces a multifocal array on the sample for parallel multiphoton excitation and then recordsfluorescence signal from all foci simultaneously with an area array detector,which significantly improves the imaging speed of multiphoton microscopy and allows for high efficiency in use of the excitation light.In this paper,we discuss the features of several MMM setups using different beamsplitting devices,including a Nipkow spinning disk,a microlens array,a set of beamsplitting mirrors,or a diffractive optical element(DOE).In particular,we present our recent work on the development of an MMM using a spatial light modulator(SLM).