Restored intestinal integrity,nutrients transporters,energy metabolism,antioxidative capacity and decreased harmful microbiota were associated with IUGR piglet's catch-up growth before weanling

作     者：Chang Cui Caichi Wu Jun Wang Ziwei Ma Xiaoyu Zheng Pengwei Zhu Nuan Wang Yuhua Zhu Wutai Guan Fang Chen Chang Cui;Caichi Wu;Jun Wang;Ziwei Ma;Xiaoyu Zheng;Pengwei Zhu;Nuan Wang;Yuhua Zhu;Wutai Guan;Fang Chen

作者机构：Guangdong Provincial Key Laboratory of Animal Nutrition ControlCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China. Shenzhen Kingsino Technology CO.LTDShenzhen 518107China. Shenzhen Institute of Nutrition and HealthHuazhong Agricultural UniversityWuhan 430070China. Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518116China. College of Animal Science and National Engineering Research Center for Breeding Swine IndustrySouth China Agricultural UniversityGuangzhou 510642China. Guangdong Laboratory for Lingnan Modern AgricultureSouth China Agricultural UniversityGuangzhou 510642China. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2023年第14卷第1期

页      面：390-408页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：the Natural Science Foundation of Guangdong Province(2021A1515010944) the National Natural Science Foundation of China(31402082 and 32272894)。 

主　　题：Antioxidative capacity Catch-up growth Gut Intrauterine growth retardation Piglets 

摘      要：Background:Intrauterine growth restriction(IUGR)is a major inducer of higher morbidity and mortality in the pig industry and catch-up growth(CUG)before weanling could significantly restore this negative influence.But there was limited knowledge about the underlying mechanism of CUG occurrence.Methods:Eighty litters of newborn piglets were divided into normal birth weight(NBW)and IUGR groups according to birth weight.At 26 d,those piglets with IUGR but over average body weight of eighty litters of weaned piglets were considered as CUG,and the piglets with IUGR still below average body weight were considered as NCUG.This study was conducted to systemically compare the intestinal difference among NBW,CUG and NCUG weaned piglets considering the crucial role of the intestine for piglet growth.Results:The results indicated that the m RNA expression of nutrients(amino acids,glucose,and fatty acids)transporters,and mitochondrial electron transport chain(ETC)I were upregulated in CUG piglets gut with improved morphology compared with those NCUG,as well as the ratio of P-AMPK/AMPK protein expression which is the indicator of energy metabolism.Meanwhile,CUG piglet s gut showed higher antioxidative capacity with increased SOD and GSHPx activity,decreased MDA levels,as well as higher m RNA expressions of Nrf2,Keap1,SOD,and GSH-Px.Furthermore,inflammatory parameters including TNF-α,IL-1β,IL-6,and IL-12 factors,and the activation of MAPK and NF-κB signaling pathways were significantly elevated in the NCUG intestine,while the protein expression of ZO-1,Occludin and Claudin-1 was reduced.The alpha diversity of fecal microbiota was higher in CUG piglets in contrast with NCUG piglets,and the increased beneficial bacteria and decreased pathogenic bacteria was also observed in CUG piglets.Conclusions:CUG piglet s intestine showed comprehensive restoration including higher nutrients transport,energy metabolism,antioxidant capacity,and intestinal physical barrier,while lower oxidative stress,inflammatory response,and pathogenic microbiota.