Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize

作     者：Huajian Gao Junjun Cui Shengxue Liu Shuhui Wang Yongyan Lian Yunting Bai Tengfei Zhu Haohao Wu Yijie Wang Shiping Yang Xuefeng Li Junhong Zhuang Limei Chen Zhizhong Gong Feng Qin Huajian Gao;Junjun Cui;Shengxue Liu;Shuhui Wang;Yongyan Lian;Yunting Bai;Tengfei Zhu;Haohao Wu;Yijie Wang;Shiping Yang;Xuefeng Li;Junhong Zhuang;Limei Chen;Zhizhong Gong;Feng Qin

作者机构：Key Laboratory of Plant Molecular PhysiologyInstitute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Plant Physiology and BiochemistryCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBejing 100193China School of Life ScienceInstitute of Life Science and Green DevelopmentHebei UniversityBaodingHebei071002China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2022年第15卷第10期

页      面：1558-1574页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：Beijing Outstanding Young Scientist Pro-gram(BJJWZYJH01201910019026) National Key Research and Devel-opment Program of China(2021YFD1200703) the National Natural Science Foundation of China(31625022,31971952). 

主　　题：ZmSRO1d stomatal ROS drought resistance yield maize 

摘      要：While crop yields have historically increased,drought resistance has become a major concern in the context of global climate change.The trade-off between crop yield and drought resistance is a common phenomenon;however,the underlying molecular modulators remain undetermined.Through genome-wide association study,we revealed that three non-synonymous variants in a drought-resistant allele of ZmSRO1d-R resulted in plasma membrane localization and enhanced mono-ADP-ribosyltransferase activity of ZmSRO1d toward ZmRBOHC,which increased reactive oxygen species(ROS)levels in guard cells and promoted stomatal closure.ZmSRO1d-R enhanced plant drought resilience and protected grain yields under drought conditions,but it led to yield drag under favorable conditions.In contrast,loss-of-function mutants of ZmRBOHC showed remarkably increased yields under well-watered conditions,whereas they showed compromised drought resistance.Interestingly,by analyzing 189 teosinte accessions,we found that the ZmSRO1d-R allele was present in teosinte but was selected against during maize domestication and modern breeding.Collectively,our work suggests that the allele frequency reduction of ZmSRO1d-R in breeding programs may have compromised maize drought resistance while increased yields.Therefore,introduction of the ZmSRO1d-R allele into modern maize cultivars would contribute to food security under drought stress caused by global climate change.