Dental stem cell-derived extracellular vesicles transfer miR-330-5p to treat traumatic brain injury by regulating microglia polarization

作     者：Ye Li Meng Sun Xinxin Wang Xiaoyu Cao Na Li Dandan Pei Ang Li Ye Li;Meng Sun;Xinxin Wang;Xiaoyu Cao;Na Li;Dandan Pei;Ang Li

作者机构：Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchCollege of StomatologyXian Jiaotong UniversityXianChina State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of EducationSchool and Hospital of StomatologyWuhan UniversityWuhanChina Department of PeriodontologyCollege of StomatologyXi'an Jiaotong UniversityXi'anChina 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2022年第14卷第4期

页      面：461-469页

核心收录：

学科分类：1003[医学-口腔医学] 1002[医学-临床医学] 10[医学] 

基　　金：supported by China Postdoctoral Innovation Science Foundation(BX20200254) China Postdoctoral Science Foundation(2021M692474) National Natural Science Foundation of China(No.81701037,82071078 and 82170927)。 

主　　题：injury transferred resume 

摘      要：Traumatic brain injury(TBI)contributes to the key causative elements of neurological deficits.However,no effective therapeutics have been developed yet.In our previous work,extracellular vesicles(EVs)secreted by stem cells from human exfoliated deciduous teeth(SHED)offered new insights as potential strategies for functional recovery of TBI.The current study aims to elucidate the mechanism of action,providing novel therapeutic targets for future clinical interventions.With the mi RNA array performed and Real-time PCR validated,we revealed the crucial function of mi R-330-5p transferred by SHED-derived EVs(SHED-EVs)in regulating microglia,the critical immune modulator in central nervous system.Mi R-330-5p targeted Ehmt2 and mediated the transcription of CXCL14 to promote M2 microglia polarization and inhibit M1 polarization.Identified in our in vivo data,SHED-EVs and their effector mi R-330-5p alleviated the secretion of inflammatory cytokines and resumed the motor functional recovery of TBI rats.In summary,by transferring mi R-330-5p,SHED-EVs favored anti-inflammatory microglia polarization through Ehmt2 mediated CXCL14transcription in treating traumatic brain injury.