Efficacy of Moxa-burning heat stimulating Zusanli(ST36) and Shenshu(BL23) on expressions of macrophage migration inhibitory factor and macrophage apoptosis in rabbits with adjuvant-induced arthritis

作     者：ZHOU Haiyan ZHONG Yumei GAO Xiuhua WU Fei JIA Min YANG Xin ZHOU Haiyan;ZHONG Yumei;GAO Xiuhua;WU Fei;JIA Min;YANG Xin

作者机构：School of Acupuncture-Moxibustion and TuinaChengdu University of Traditional Chinese MedicineChengdu 610075China Pain DepartmentChengdu First People's Hospital/Chengdu Integrated TCM&Western Medicine HospitalChengdu 610095China Centre of Preventive Treatment of DiseaseTeaching Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610075China Foreign Languages SchoolChengdu University of Traditional Chinese MedicineChengdu 610075China Acupuncture DepartmentSecond Affiliated Hospital of Baotou Medical CollegeBaotou 014030China Health Rehabilitation SchoolChengdu University of Traditional Chinese MedicineChengdu 610075China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第6期

页      面：980-987页

核心收录：

学科分类：1005[医学-中医学] 1002[医学-临床医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China:Study on the Immune Mechanisms of Macrophage M1/M2 Polarization in the Treatment of Rheumatoid Arthritis by Moxibustion “Strengthening Body Resistance and Eliminating Evil”(No. 81973959) National Natural Science Foundation of China:Research on “ImmuneInflammation” Molecular Signal Regulation of NLRP3 Inflammasomes in RA with Moxibustion Treatment (No. 81774435) National Key R&D Program of China:Research on the Functional Characterics of “Special Effect” and “Common Effect” of Acupoints (No. 2019YFC1709001) Foundation of Sichuan Provincial Administration of Traditional Chinese Medicine:Research on MIF-GC Rhythm Regulation Mechanism of Moxibustion in the Treatment of Rheumatoid Arthritis (No. 2018JC007) Foundation of Chengdu University of Traditional Chinese Medicine:Research on the Mechanism of “MIF-target Protein-GC-inflammation” of Antiinflammatory Effect of Moxibustion on Rheumatoid Arthritis (No. QNXZ2018034)。 

主　　题：rheumatoid arthritis moxibustion macrophage migration-inhibitory factors macrophages 

摘      要：OBJECTIVE: To evaluate the effects of moxa-burning heat stimulating acupoints Zusanli(ST36) and Shenshu(BL23) on macrophage migration inhibitory factor(MIF) and its related molecules which can provide scientific experimental basis for the clinical application of moxibustion treatment of rheumatoid arthritis(RA). METHODS: Thirty rabbits were randomly assigned to control group, RA model(established by injecting Freund’s Complete Adjuvant) group(RA group) and RA model with moxibustion group [Moxa group, Zusanli(ST36) and Shenshu(BL23), 5 moxa pillars/day, 6 d × 3]. The expressions of MIF m RNA were evaluated with reverse transcription polymerase chain reaction;the apoptosis rates of macrophages were detected by erminal deoxynucleotidyl transferase-mediated d TUP nick end labeling;the expressions of related signal molecules were detected with immunohistochemical S-P method and the levels of IL-2 were detected with enzymelinked immunosorbent assay. RESULTS: The expressions of MIF m RNA, extracellular regulated protein kinases 2, p38 mitogen-activated protein kinase and nuclear factor-κ-gene binding p65 in synovial tissue of RA group were significantly increased when compared with control group, which were lower remarkably in moxa group than those in RA group. The apoptosis rates of macrophages in RA group were significantly down-regulated as compared with the control group, which were up-regulated in moxa group compared with the RA group. The levels of IL-2 in synovial fluid from the RA group were elevated significantly as compared with that from control group, but those of the moxa group were reduced when compared with those from RA group. CONCLUSIONS: Moxibustion may simultaneously regulate the expressions of MIF and its related signaling pathways molecules, the apoptosis rate of macrophages in synovial tissue, as well as the level of inflammatory factors in synovial fluid. The results suggest that the antiinflammatory effect of moxibustion on RA may be related to inhibit the expression of MIF in synovial tissue, the molecules of some related signaling pathways and promote the apoptosis of macrophage.