Analysis of the linkage positions and isomers of monosaccharide units in oligosaccharides by negative secondary ion mass spectrometry in combination with the stereoselective reaction with substituted boronic acid

作     者：YAN,Lin FANG,Yi-Wei Institute of Chemistry,Chinese Academy of Sciences,Beijing 100080 

作者机构：Institute of Chemistry Chinese Academy of Sciences Beijing 100080 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：1993年第11卷第1期

页      面：53-58页

核心收录：

学科分类：07[理学] 0703[理学-化学] 

基　　金：Project supported by the National Natural Science Foundation of China 

主　　题：Analysis of the linkage positions and isomers of monosaccharide units in oligosaccharides by negative secondary ion mass spectrometry in combination with the stereoselective reaction with substituted boronic acid acid 

摘      要：The negative secondary ion mass spectrometry,in combination with the stereoselective derivatizations with substituted boronic acid RB(OH)_2,was used in the analysis of fourteen oligosac- *** mass spectra of the derivatives provide information on their linkage Positions and iso- merism of the individual monoscaccharide *** results indicated that among the derivatives of the oligosaccharides analyzed,those with 1—4 and 1—6 linkages all presented the ion peaks at m/z 287,sometimes one more peak at m/z ***,a relationship was found between the linkage positions and the intensity orders of the derivative ***,the derivatives of the disaccharides with a galactose presented an intense ion peak at m/z 347,and those of oligosaccharides with 1—6 linkage to a galactose at terminal presented the ion at m/z *** the case of oligosaccharides with a fructose residue,characteristic ion of m/z 155 was *** conditions of stereoselective derivatizations and mass spectrometry were studied,in order to obtain a better reproducibility of the mass spectra.