Hsa_circ_0001658 accelerates the progression of colorectal cancer through miR-590-5p/METTL3 regulatory axis

作     者：Yang Lu Xing-Ming Wang Ze-Shu Li Ai-Juan Wu Wen-Xia Cheng 

作者机构：Department of OncologyPKUCare Luzhong HospitalZibo 255400Shandong ProvinceChina Department of OncologyZibo Maternal and Child Health HospitalZibo 255095Shandong ProvinceChina 

出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)

年 卷 期：2023年第15卷第1期

页      面：76-89页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Circ_0001658 miR-590-5p METTL3 Colorectal cancer Proliferation 

摘      要：BACKGROUND As reported,multiple circular RNAs(circRNAs)interfere with colorectal cancer(CRC)***,circRNA_0001658(circ_0001658)is focused on studying how it works in *** Clarify the expression pattern,biological function,and underlying mechanism of circ_0001658 of CRC *** In CRC-related chip data retrieved using the database named Gene Expression Omnibus,different expressions of circRNAs between CRC and normal tissue samples were *** Real-time PCR and Western blot ensured the analysis on circ_0001658,microRNA-590-5P(miR-590-5p),and methyltransferase-like 3(METTL3)mRNA expressions in tissues and *** counting kit-8 and flow cytometry were used to detect cell proliferation,apoptosis and *** targeting relations between circ_0001658,miR-590-5p,and METTL3 mRNA 3 -untranslated region were under the verification of bioinformatics prediction and dual luciferase-based reporter gene *** Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were employed on the downstream targets of miR-590-5p using the Database for Annotation,Visualization and Integrated Discovery *** Circ_0001658 and METTL3 mRNA was elevated in CRC tissues and cells,whereas miR-590-5p was ***_0001658 overexpression promoted the proliferation of HT29 cells,inhibited apoptosis,and accelerated the cell *** SW480 cells,knocking down circ_0001658 had the opposite ***_0001658 could specifically bind to miR-590-5p and negatively modulate its expressions;METTL3 is a miR-590-5p target that can be positively regulated by circ *** 0001658 was inversely associated with miR-590-5p expression while positively with METTL3 *** Circ_0001658 regulates the miR-590-5p/METTL 3-axis to increase CRC cell growth and decrease apoptosis.