Importance of adapted digestion conditions to simulate in vitro lipid digestion of broilers in different life stages

作     者：Daphne Michels Sarah H.E.Verkempinck Agnese Panozzo Karen Vermeulen Marc E.Hendrickx Liesbet Thijs Tara Grauwet Daphne Michels;Sarah H.E.Verkempinck;Agnese Panozzo;Karen Vermeulen;Marc E.Hendrickx;Liesbet Thijs;Tara Grauwet

作者机构：Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre(LFoRCe)Department of Microbial and Molecular Systems(M2S)KU LeuvenLeuven3001Belgium Kemin Animal Nutrition and HealthKemin Europa N.V.Herentals2200Belgium 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第12卷第1期

页      面：151-158页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：D.Michels is a Doctoral Researcher funded by VLAIO(Flanders Innovation and Entrepreneurship,Belgium)in the context of a Baekeland mandate(HBC.2020.2836) S.H.E.Verkempinck is a Postdoctoral Researcher funded by the Research Foundation Flanders(FWO–Grant no.1222420N) 

主　　题：Lipolysis Lipase activity Bile salt In vitro digestion Broiler chicken Emulsion 

摘      要：In vitro digestion studies demonstrate large potential to gain more and quicker insights into the underlying mechanisms of feed additives,allowing the optimization of feed ***,current in vitro digestion models relevant for broiler chickens lack sufficient description in terms of protocols and standardisation ***,no distinction is made between the different life phases of these animals(starter,grower,and finisher).Hence,our research aimed to establish adapted in vitro digestion conditions,corresponding to the 3 life phases in broilers,with specific focus on lipid *** effect of 3 different bile salt concentrations of 2,10,and 20 mM,and 3 different lipase activities of 5,20,and 100 U/mL,on in vitro lipid digestion kinetics were evaluated using a full factorial *** values were selected to represent starter,grower,and finisher birds,*** findings showed that the extent of lipid digestion was mainly influenced by lipase *** rate of lipid digestion was affected by an interplay between bile salt concentration and lipase activity,due to possible lipase inhibition at certain bile salt ***,this work resulted in 3 in vitro lipid digestion models representative for starter,grower,and finisher *** conclusion,this research showed the impact of adapted in vitro digestion conditions on lipid digestion kinetics and thus the need for these conditions relevant for each life phase of broilers.