Expression of Nitric Oxide Synthase Isoenzyme in Lung Tissue of Smokers with and without Chronic Obstructive Pulmonary Disease

作     者：Wen-Ting Jiang Xian-Sheng Liu Yong-Jian Xu Wang Ni Shi-Xin Chen Jiang Wen-Ting;Liu Xian-Sheng;Xu Yong-Jian;Ni Wang;Chen Shi-Xin

作者机构：Department of Respiratory Medicine Tongji Hospital Affiliated to Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2015年第128卷第12期

页      面：1584-1589页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：This work was supported by the grants from Natural Science Foundation of China (No. 81270106)  Ministry of Science and Technology of China (No. 2012BA[05B00) and Ministry of Public Health (No. 201002008) 

主　　题：Chronic Obstructive Pulmonary Disease Nitric Oxide Synthase Smoking 

摘      要：Background：It has been demonstrated that only 10%-20% cigarette smokers finally suffer chronic obstructive pulmonary disease （COPD）.The underlying mechanism of development remains uncertain so *** oxide （NO） has been found to be closely associated with the pathogenesis of COPD,the alteration of NO synthase （NOS） expression need to be *** study aimed to investigate the alterations of NOS isoforms expressions between smokers with and without COPD,which might be helpful for identifying the susceptibility of smokers developing into ***：Peripheral lung tissues were obtained from 10 nonsmoker control subjects,15 non-COPD smokers,and 15 smokers with *** NOS （nNOS）,inducible NOS （iNOS）,and endothelial NOS （eNOS） mRNA and protein levels were measured in each sample by using real-time polymerase chain reaction and Westem ***：INOS mRNA was significantly increased in patients with COPD compared with nonsmokers and smokers with normal lung function （P 〈 0.001,P =0.001,respectively）.iNOS protein was also higher in COPD patients than nonsmokers and smokers with normal lung function （P 〈 0.01 and P =0.01,respectively）.However,expressions ofnNOS and eNOS did not differ among nonsmokers,smokers with and without ***,there was a negative correlation between iNOS protein level and lung function parameters forced expiratory volume in 1 s （FEV1） （% predicted） （r =-0.549,P =0.001） and FEV1/forced vital capacity （%,r =-0.535,P =0.001）.Conclusions：The expression of iNOS significantly increased in smokers with COPD compared with that in nonsmokers or smokers without *** results suggest that iNOS might be involved in the pathogenesis of COPD,and may be a potential marker to identify the smokers who have more liability to suffer COPD.