Functional electrical stimulation increases neural stem/progenitor cell proliferation and neurogenesis in the subventricular zone of rats with stroke

作     者：LIU Hui-hua XIANG Yun YAN Tie-bin TAN Zhi-mei LI Sheng-huo HE Xiao-kuo 

作者机构：Department of Rehabilitation Medicine bun Yat-sen lviemorlal Hospital Sun Yat-sen University Guangzhou Guangdong 510120 China Department of Rehabilitation Medicine Sixth People's Hospital of Shenzhen Shenzhen Guangdong 518052 China Department of Rehabilitation Medicine First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450000 China Department of Rehabilitation Medicine Second Affiliated Hospital of Guangzhou Medical College Guangzhou Guangdong 510260 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2013年第126卷第12期

页      面：2361-2367页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by China’s National Natural Science Research Council 

主　　题：functional electrical stimulation subventricular zone ischemia neurogenesis 

摘      要：Background Functional electrical stimulation （FES） is known to promote the recovery of motor function in rats with ischemia and to upregulate the expression of growth factors which support brain *** this study,we investigated whether postischemic FES could improve functional outcomes and modulate neurogenesis in the subventricular zone （SVZ） after focal cerebral *** Adult male Sprague-Dawley rats with permanent middle cerebral artery occlusion （MCAO） were randomly assigned to the control group,the placebo stimulation group,and the FES *** rats in each group were further assigned to one of four therapeutic periods （1,3,7,or 14 days）.FES was delivered 48 hours after the MCAO procedure and divided into two 10-minute sessions on each day of treatment with a 10-minute rest between *** intraperitoneal injections of bromodeoxyuridine （BrdU） were given 4 hours apart every day beginning 48 hours after the *** was evaluated by immunofluorescence ***-3 which is strongly implicated in the proliferation and differentiation of neural stem cells （NSCs） was investigated by Western blotting *** data wera subjected to oneway analysis of variance （ANOVA）,followed by a Tukey/Kramer or Dunnett post hoc *** FES significantly increased the number of BrdU-positive cells and BrdU/glial flbrillary acidic protein doublepositive neural progenitor cells in the SVZ on days 7 and 14 of the treatment （P 〈0.05）.The number of BrdU/doublecortin （DCX） double-positive migrating neuroblast cells in the ipsilateral SVZ on day 14 of the FES treatment group （（522.77±33.32） cells/mm2） was significantly increased compared with the control group （（262.58±35.11） cells/mm2,P 〈0.05） and the placebo group （（266.17±47.98） cells/mm2,P 〈0.05）.However,only a few BrdU/neuron-specific nuclear protein-positive cells were observed by day 14 of the *** day 7,Wnt-3 was upregulated in the ipsilateral SVZs of the rats receiving FES （（0.44±0