Normal and reverse flow injection-spectrophotometric determination of thiamine hydrochloride in pharmaceutical preparations using diazotized metoclopramide

作     者：Mouayed Q.Al Abachi Hind Hadi 

作者机构：Department of ChemistryCollege of ScienceUniversity of BaghdadJadriyahBaghdadIraq 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2012年第2卷第5期

页      面：350-355页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0703[理学-化学] 10[医学] 0702[理学-物理学] 

基　　金：We would like to thank the Department of Chemistry at University of Baghdad and the industries and Medical Appliance  SDI  Samara  Iraq 

主　　题：Thiamine Metoclopramide Flow injection Spectrophotometry 

摘      要：Simple and sensitive normal and reverse flow injection methods for spectrophotometric determination of thiamine hydrochloride （THC） at the microgram level were proposed and optimized. Both methods are based on the reaction between THC and diazotized metoclopramide in alkaline medium. Beer＇s law was obeyed over the range of 10 300 and 2-90 ug/mL, the limits of detection were 2.118 and 0.839 ug/mL and the sampling rates were 80 and 95 injections per hour for normal and reverse flow injection methods respectively. The application of both methods to commercially available pharmaceuticals produced acceptable results. The flow system is suitable for application in quality control processes.