Ultrasensitive and accurate diagnosis of urothelial cancer by plasmonic AuNRs-enhanced fluorescence of near-infrared Ag_(2)S quantum dots

作     者：Dong Zhang Cai-Ping Ding Xiao-Yue Zheng Jia-Zhou Ye Zi-Hai Chen Jian-Hua Li Ze-Jun Yan Jun-Hui Jiang You-Ju Huang Dong Zhang;Cai-Ping Ding;Xiao-Yue Zheng;Jia-Zhou Ye;Zi-Hai Chen;Jian-Hua Li;Ze-Jun Yan;Jun-Hui Jiang;You-Ju Huang

作者机构：Department of Urology&NephrologyNingbo First HospitalThe Affiliated Hospital of Zhejiang UniversityNingbo315000China College of MaterialChemistry and Chemical EngineeringKey Laboratory of Organosilicon Chemistry and Material TechnologyMinistry of EducationHangzhou Normal UniversityHangzhou311121China Anhui Shenghaitang Traditional Chinese Medicine Decoction Pieces Co.LtdBozhou236800China Anhui Topway Testing Services Co.Ltd.Xuancheng Economic and Technological Development ZoneXuancheng242000China 

出 版 物：《Rare Metals》 (稀有金属（英文版）)

年 卷 期：2022年第41卷第11期

页      面：3828-3838页

核心收录：

学科分类：1002[医学-临床医学] 07[理学] 0806[工学-冶金工程] 070302[理学-分析化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 

基　　金：financially supported in part by the National Natural Science Foundation of China(Nos.22005081,51873222 and 52111530128) Zhejiang Provincial Natural Science Foundation of China(Nos.LY22B050003 and LZ22B050001) the Funding for the Scientific Research Foundation for Scholars of Hangzhou Normal University(Nos.4095C5021920467 and 4095C5021920452) the Key Research and Development Projects of Anhui Province(Nos.202004g01020016 and 202104g01020009)。 

主　　题：Urothelial carcinoma FXYD3 Plasmonic enhanced fluorescence Ultrasensitive Accurate diagnosis 

摘      要：Urothelial carcinoma(UC)is a common malignant tumor in the urinary system with high recurrence rate and low survival rate 5 years after surgery.At present,imaging examination and other diagnostic methods have some shortcomings such as invasiveness and non-specificity.Therefore,it is urgent to develop a simple,rapid,noninvasive,highly sensitive and highly specific strategy to diagnose UC.Herein,a high-performance fluorescence sensor was constructed by the plasmonic gold nanorods(AuNRs)-enhanced near-infrared(NIR)fluorescence of silver sulfide quantum dots(Ag_(2)S QDs).The designed sensor can be used for the fast and accurate detection of small molecule single-transmembrane protein(FXYD3),which is overexpressed in 90%of ureteral cancers and 84%of high-grade bladder cancers.Due to its high specificity,the NIR fluorescence sensor achieves the detection of FXYD3 in the range of 0.25-150 ng·ml^(-1)with a detection limit of 0.2 ng·ml^(-1).Importantly,it also can be used for accurate diagnosis of FXYD3 in the urine of patients with relevant cancers,and the results are consistent with clinical cystoscopy and pathological analysis.The proposed fluorescence sensor provides a simple,ultrasensitive,reliable method for UC screening,tumor-grade classification and postoperative monitoring and will have great potential for clinical applications.