A female in vivo haploid-induction system via mutagenesis of egg cell-specific peptidases
作者机构：State Key Laboratory of Hybrid RiceCollege of Life SciencesWuhan UniversityWuhan 430072China Hubei Hongshan LaboratoryWuhan 430070China Cell Biology and Plant BiochemistryUniversity of RegensburgRegensburgGermany
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第2期
页 面：471-480页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by the National Natural Science Foundation of China(grants 32130031 and 32000248) the Major Project of Hubei Hongshan Laboratory(2022hszd017) the China Postdoctoral Science Foundation(grants 2021M702525 and BX20200256)
主 题：haploid induction egg cell maternal inducer semigamy fertilization
摘 要：Crop breeding schemes can be significantly accelerated by using(doubled)haploid *** vivo haploid induction has been applied in plant breeding for decades but is still not available for all crops and genotypes,and haploidization rates are generally very ***,methodological improvements to and new concepts for haploidization are ***,we report a novel system for the induction of haploid plants by mutating genes encoding egg cell-specific aspartic endopeptidases(ECSs).We show that after successful sperm–egg cell fusion,ECSs play a critical role to ensure male and female nucleus fusion after *** ecs1 ecs2 double mutant can induce haploids by both selfing and hybridization in Arabidopsis and ECS mutation is also capable of producing haploids in *** summary,our study develops a novel approach for maternal haploidization and provides new insights into the molecular basis of fertilization.
维普期刊数据库