Bioactive 3D-printed chitosan-based scaffolds for personalized craniofacial bone tissue engineering

作     者：Satar Yousefiasl Esmaeel Sharifi Erfan Salahinejad Pooyan Makvandi Soussan Irani 

作者机构：School of DentistryHamadan University of Medical SciencesHamadan ***Iran Department of Tissue Engineering and BiomaterialsSchool of Advanced Medical Sciences and TechnologiesHamadan University of Medical SciencesHamadanIran Faculty of Materials Science and EngineeringK.N.Toosi University of TechnologyTehranIran Centre for Materials InterfacesIstituto Italiano di Tecnologiaviale Rinaldo Piaggio 34PontederaPisa 56025Italy Dental Research CentreOral Pathology DepartmentDental FacultyHamadan University of Medical SciencesHamadanIran 

出 版 物：《再生工程(英文)》 (Engineered Regeneration)

年 卷 期：2023年第4卷第1期

页      面：1-11页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：Vice Chancellor for Research and Technology  Hamadan University of Medical Sciences 

主　　题：Bioactivity CAD/CAM Maxillofacial reconstruction Pore Stem cells 

摘      要：Regeneration of craniofacial bone defects is a key issue in the bone regeneration ***,novel treatment strategies,such as tissue engineering using porous scaffolds,have been *** ideal tissue-engineered scaffold for bone tissue regeneration should possess pores to facilitate nutrients transmission and support repar-ative tissue ingrowth,bioactivity for osteoconduction and osseointegration,and biocompatibility to improve cell attachment,proliferation,and extracellular matrix *** the present study,we manufactured chitosan-based hydrogels substituted with alginate with optimized properties by extrusion-based three-dimensional(3D)printing.3D printing of the scaffolds enables the designing and developing of complex architectures for cranio-facial reconstruction using computer-aided design(CAD).Different ratios(2.5,5,and 10%)of hydroxyapatite were added to the hydrogel,printed,and subsequently lyophilized to augment the physical and biological char-acteristics of the *** incorporation into the chitosan-based scaffolds increased the porosity and pore size of the printed *** addition,the presence of hydroxyapatite amplified apatite formation and decreased the size of formed apatite *** the scaffold samples showed biocompatible properties and did not have toxicity toward rat bone marrow mesenchymal stem ***,the scaffolds containing 5%w/w hydroxyapatite exhibited significant growth in cell viability compared to the ***,it is concluded that chitosan-based scaffolds adorned with hydroxyapatite are considerable for regenerating craniofacial bone defects.