Diagnostic Value of Hepatic Enzymes, Triglycerides and Serum Proteins for the Detection of Hepatic Lipidosis in Chelonoidis carbonaria in Captivity

作     者：Gustavo Henrique Pereira Dutra 

作者机构：Aquario Municipal de Santos Avenida Bartolomeu de Gusmao s/n Ponta da Praia-Santos-SP 11030-500 Brazil 

出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)

年 卷 期：2014年第8卷第8期

页      面：633-639页

学科分类：090603[农学-临床兽医学] 081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0906[农学-兽医学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

主　　题：谷草转氨酶 肝脏脂质 脂肪沉积 甘油三酯 诊断价值 象龟 血清蛋白 南美 

摘      要：Hepatic lipidosis is a common lesion in chelonians and may be related with vitellogenesis, hyperparathyroidism, follicular stasis, fatty diets or lacking nutrients, sedentary life and obesity, bacterial toxins in liver and starvation. Clinical signs are unspecific. Routine biochemical tests dont have specificity and it is better defined through biopsy. The objective was evaluated the biochemical hepatic profile of red footed tortoises (Chelonoidis carbonaria) with the histopathological findings of lipidosis by laparoscopic biopsy. Samples of blood were collected for biochemistry of thirty-six animals from two different groups, twenty animals from a zoo and sixteen from a breeder. The animals were submitted to liver biopsy by videolaparoscopy. Three fragments were collected, two of them were processed by histology and one went to electron microscopy. Nineteen tortoises from the zoo had lipidosis in laparoscopy and macrovesicular degeneration. Fifteen tortoises from the breeder had microvesicular degeneration. The diagnosis of steatosis was confirmed by electron microscopy. There was no statistical difference of aspartate aminotransferase levels between the two groups; however there was significant difference of triglycerides levels. The enzymatic activity of transaminases is not related to the histological grading of lipidosis in tortoises, however triglycerides levels are higher in animals with more severe gradation.