Rapid quantitative analysis of 12 chemical constituents in wild-simulated and cultivated Astragali Radix based on UHPLC-MS

作     者：Dan Li Yuetao Liu Xuemei Qin Dan Li;Yuetao Liu;Xuemei Qin

作者机构：Modern Research Center for Traditional Chinese MedicineKey Laboratory of Chemical Biology and Molecular Engineering of Ministry of EducationShanxi UniversityTaiyuan 030006China Shanxi Key Laboratory of Effective Substances Research and Utilization in Traditional Chinese MedicineTaiyuan 030006China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2022年第14卷第3期

页      面：464-469页

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 10[医学] 100602[医学-中西医结合临床] 

基　　金：financially supported by National Natural Science Foundation of China (No.31770362). 

主　　题：Astragali Radix chemical constituent cultivation method quantification UHPLC-MS 

摘      要：Objective:Astragali Radix(AR)is one of the most widely used traditional Chinese medicines(TCMs)for tonic,which can be divided into wild-simulated and cultivated AR according to its cultivation method.However,whether cultivated AR can replace wild-simulated AR has always been a concern.Methods:In this study,a rapid,highly sensitive and specific analytical method using ultra-high performance liquid chromatography tandem mass spectrometry(UHPLC-MS)was developed to quantitatively measure 12 chemical constituents of AR in the different cultivation methods.Results:AR samples were analyzed with a good linear regression relationship(R2,0.9983–0.9995),precisions(relative standard deviation(RSD),1.31%-2.36%),repeatability(RSD,2.65%-4.92%),stability(RSD,1.50%-4.05%),and recovery(95.13%-106.52%).Through the determination of AR samples,we found the components of favonoids in wild-simulated AR were higher than cultivated AR,the saponins in cultivated AR were higher,the ratio of saponins/favonoids in cultivated AR was higher than wildsimulated AR.Conclusion:Based on this research,it could provide guidance for the quality control of AR.