Engineering crop Phytophthora resistance by targeting pathogen-derived PI3P for enhanced catabolism
作者机构：Key Laboratory of Plant ImmunityCollege of Plant ProtectionAcademy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095China Institute of Industrial CropsJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing 210095China College of Plant ProtectionChina Agricultural UniversityBeijing 100091China Crops Research InstituteGuangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic ImprovementGuangdongGuangzhou 510640China Department of Plant PathologyWashington State UniversityPullmanWA 99164USA
出 版 物：《Plant Communications》 (植物通讯（英文）)
年 卷 期：2023年第4卷第2期
页 面：167-178页
核心收录：
基 金：supported by the National Natural Science Foundation of China,China(32072507,32272495,and 31721004) the Natural Science Foundation of Jiangsu Province,China(BK20220147)
主 题：AtPIP5K1 Phytophthora resistance PI3P RxLR effector transgenic plant
摘 要：Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by *** and soybean root rot caused by *** previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate(PI3P)to promote infection via direct association with RxLR ***,we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1(AtPIP5K1),which can phosphorylate PI3P to PI(3,4)*** fused AtPIP5K1 with the soybean PR1a signal peptide(SP-PIP5K1)to enable its secretion into the plant *** soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various *** and *** isolates,***-PIP5K1 significantly reduced PI3P accumulation during *** and soybean *** or inhibition of PI3 kinases(PI3Ks)in *** compromised the resistance mediated by SP-PIP5K1,indicating that SP-PIP5K1 action requires a supply of pathogen-derived ***,we revealed that SP-PIP5K1 can interfere with the action of *** mediated by the RxLR effector *** novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.
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