Enhanced expression of the decoy receptor IL-13Rα2 in macrophages of Schistosomajaponicum-infected mice

作     者：WANG Wei SHEN Yu-xian LI Jing ZHANG Shi-hai LUO Qing-li ZHONG Zhen-rong JIANG Zuo-jun SHEN Ji-long 

作者机构：Department of Epidemiology and Biostatistics School of Public Health Department of Pathobiology Anhui Medical University Hefei Anhui 230032 China Key Laboratory of Gene Resource Utilization for Severe Diseases the Ministry of Education of China and Anhui Province Hefei 230032 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2009年第122卷第14期

页      面：1650-1654页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：This work was supported by Natural Science Foundation of Anhui Educational Committee (No. KJ2009A80) 

主　　题：schistosomiasis macrophages interleukin-13R 

摘      要：Background Type 2 cytokine interleukin （IL）-13 and its decoy receptor, IL-13 receptor （R）a2 appear to play a major role in tissue fibrosis of schistosomiasis and asthma. IL-13 is a key regulator of the extracellular matrix （ECM）. It is known to signal to cells by binding to the IL-13Rα1, which then heterodimerizes with IL-4Rα. In contrast, IL-13Rα2 binds IL-13 with high affinity but does not signal. IL-13Rα2 is known to down-regulate granulomatous inflammation and prolong host survival in Schistosoma mansoni （S. mansoni） infection, but little is known about the location and expression level of IL-13Rα2 in the context of S. japonicum infection. Methods We established S. japonicum-infected mouse models. Kinetic serum levels of IL-13Rα2 were examined with ELISA. IL-13Rα2 mRNA and protein of liver tissues were determined by PCR and immunoblotting analysis, respectively. Detection of IL-13Rα2 expression and location in macrophages was performed by TaqMan PCR and fluorescent immunocytochemistry technique, respectively. Results A marked elevation of mRNA and protein expression of IL-13Rα2 was observed in mice during S. japonicum infection. An enhanced expression of IL-13Rα2 was further demonstrated in primary macrophages of murine schistosomiasis. Conclusions IL-13Rα2 in macrophages may be a critical contributor to pathogenesis of schistosomiasis. The data highlight the potential importance of cell signaling and antifibrotic gene therapeutics in T helper 2 cell （Th2）-mediated diseases.