Phytochemical Analysis,Estimation of Quercetin,and in Vitro Anti-Diabetic Potential of Stevia Leaves Samples Procured from Two Geographical Origins
作者机构：Department of Pharmacognosy and PhytochemistryKrupanidhi College of PharmacyVarthurHobliBengaluru560035India Department of Pharmacy PracticeCollege of PharmacyAlMaarefa UniversityDariyahRiyadh13713Saudi Arabia Department of Clinical Laboratory SciencesThe Faculty of Applied Medical SciencesTaif UniversityTaif21944Saudi Arabia Centre of Biomedical Sciences Research(CBSR)Deanship of Scientific ResearchTaif UniversityTaif21944Saudi Arabia Department of Pharmaceutical SciencesCollege of Clinical PharmacyKing Faisal UniversityAl-HofufAl-Ahsa31982Saudi Arabia Department of PharmaceuticsVidya Siri College of PharmacyBangalore560035India Department of Biotechnology and Food ScienceFaculty of Applied SciencesDurban University of TechnologyDurban4001South Africa
出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)
年 卷 期：2022年第91卷第10期
页 面：2349-2365页
核心收录：
学科分类：0710[理学-生物学] 1006[医学-中西医结合] 10[医学] 100602[医学-中西医结合临床]
基 金：Walaa F.Alsanie would like to acknowledge Taif University for support No.TURSP(2020/53) Syed Mohammed Basheeruddin Asdaq,would like to thank AlMaarefa University,Riyadh,Saudi Arabia for extending support(TUMA-2021-1)to do this research.
主 题：Antidiabetic activity Stevia rebaudiana phytochemicals proximate analysis TLC RP-HPLC
摘 要：The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert.(SRB)leaves collected from two different geographical sources.SRB leaves were collected from Bangalore,Karnataka,India,and Reduit,Mauritius.Extracts were prepared using ethanol and aqueous solvents.Proximate analysis was used to evaluate moisture content,ash values,crude fibers,and extractive values.Following that,preliminary phytochemical screening was done on both ethanol leaves extracts,and subsequently total flavonoid content was determined.In addition,TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence of flavonoid components in both leaves extracts,followed by in vitro anti-diabetic activity was performed with alpha amylase and alpha glucosidase enzymes against Acarbose as standard.Results revealed that both the extracts from two different geographical sources varied significantly with the yield,content of chemicals,and presence of quercetin(flavonoid)content when estimated through the RP-HPLC standardized method.Glycosides,flavonoids,proteins,steroids,saponins,terpenoids,and phenols were found in various concentrations during phytochemical screening.Among both zones,the ethanol leaves extract of SRB taken from Mauritius had a greater content of phytochemicals and a higher yield than other extracts due to the soil nature.The Mauritius sample had greater total flavonoid levels as well as more quercetin(0.92±0.011)than the other extracts.Following that,ethanol extract inhibited enzymes(alpha amylase,alpha glucosidases)more than aqueous extract,and this inhibition was dose dependent.Among them,the Mauritius ethanol sample showed higher anti-diabetic efficacy than the Indian sample,but this difference was not significant.Overall,SRB ethanol leaves extracts outperformed other leaves extracts in terms of yield,phytoconstituents,and total flavonoids.Overall,both SRB samples had high quercetin levels and possessed anti-diabetic potential,but they were greater in the Mauritius sample,demonstrating that plant traits are influenced by geographic location.
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