Multiplexed Superresolution CRISPR Imaging of Chromatin in Living Cells

作     者：Shaopeng Wang Yaya Hao Luhao Zhang Fei Wang Jiang Li Lihua Wang Chunhai Fan 

作者机构：Division of Physical Biology and Bioimaging CenterShanghai Synchrotron Radiation FacilityCAS Key Laboratory of Interfacial Physics and TechnologyShanghai Institute of Applied PhysicsUniversity of the Chinese Academy of SciencesChinese Academy of SciencesShanghai 201800China School of Chemistry and Chemical Engineeringand Institute of Molecular MedicineRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200240China 

出 版 物：《CCS Chemistry》 (中国化学会会刊（英文）)

年 卷 期：2019年第1卷第3期

页      面：278-285页

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This work was supported by the National Key R&D Program of China(2016YFA0400900) National Natural Science Foundation of China(21775157,21390414,61475181,and U1632125) Key Research Program of Frontier Sciences,Chinese Academy of Sciences(QYZDJ-SSW-SLH031) 

主　　题：CRISPR chromatin imaging multicolor cell imaging superresolution 

摘      要：Visualization of the spatiotemporal organization of chromatin is highly desirable in the study of genome function *** regularly interspa-ced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease system has shown great promise for application in real-time chromatin imag-ing due to its DNA targeting ability in living *** studies typically used fluorescent proteins to generate fluorescent signals which,however,have trade-offs among signal intensity,multiplexibility,and simplicity.