Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis

作     者：Hameed Akbar Jun Cao Dongmei Wang Xiao Yuan Manjuan Zhang Saravanakumar Muthusamy Xiaoyu Song Xu Liu Felix Aikhionbare Xuebiao Yao Xinjiao Gao Xing Liu Hameed Akbar;Jun Cao;Dongmei Wang;Xiao Yuan;Manjuan Zhang;Saravanakumar Muthusamy;Xiaoyu Song;Xu Liu;Felix Aikhionbare;Xuebiao Yao;Xinjiao Gao;Xing Liu

作者机构：MOE Key Laboratory for Cellular Dynamics and School of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China CAS Center for Excellence in Molecular and Cell SciencesAnhui Key Laboratory for Cellular Dynamics and Chemical BiologyHefei 230027China Morehouse School of MedicineAtlantaGA 30310USA 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2022年第14卷第8期

页      面：53-66页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by grants from the National Key Re-search and Development Program of China(2017YFA0503600 and 2016YFA0100500) the National Natural Science Founda-tion of China(31621002,32090040,91854203,21922706,91853115,92153302,22177106,92053104,31970655,and 32100612) the Ministry of Education(IRT_17R102),Anhui Provincial Natural Science Foundation(2108085J15) the Strate-gic Priority Research Program of the Chinese Academy of Sci-ences(XDB19040000) the Fundamental Research Funds for the Central Universities(WK2070000066 and WK2070000194) 

主　　题：mitosis spindle Nup62 TIP60 acetylation 

摘      要：Stable transmission of genetic information during cell division requires faithful mitotic spindle assembly and chromosome *** eukaryotic cells,nuclear envelope breakdown(NEBD)is required for proper chromosome *** a list of mitotic kinases has been implicated in NEBD,how they coordinate their activity to dissolve the nuclear envelope and protein machinery such as nuclear pore complexes was ***,we identified a regulatory mechanism in which Nup62 is acetylated by TIP60 in human cell ***62 is a novel substrate of TIP60,and the acetylation of Lys432 by TIP60 dissolves nucleoporin Nup62-Nup58-Nup54 complex during entry into ***,this acetylation-elicited remodeling of nucleoporin complex promotes the distribution of Nup62 to the mitotic spindle,which is indispensable for orchestrating correct spindle ***,suppression of Nup62 perturbs accurate chromosome segregation during *** results establish a previously uncharacterized regulatory mechanism in which TIP60-elicited nucleoporin dynamics promotes chromosome segregation in mitosis.