The application of CRISPR/Cas technologies to Brassica crops:current progress and future perspectives
作者机构：State Key Laboratory of North China Crop Improvement and RegulationCllge of Life SciencesHebei Agricultural UniversityBaoding 071001China Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei ollaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaoding 071001China
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2022年第3卷第2期
页 面：146-161页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 0836[工学-生物工程]
基 金：supported by the Key Research and Development Program of Hebei(21372901D,216Z2904G,21326344D) Hebei Provincial Natural Science Foundation for Excellent Young Scholar(C2020204062) Hebei Innovative Research_Group,Project(C202020411) Program for Young Talents of Hebei Education Department(BJ2021025) International Science and Technology Cooperation base Special Project of Hebei(20592901D) Starting Grant from Hebei Agricultural University(YJ201958).
主 题：CRISPR Cas9 Cas12 Precise genome editing Brassica
摘 要：Brassica species are a global source of nutrients and edible vegetable oil for humans.However,all commercially important Brassica crops underwent a whole-genome triplication event,hindering the development of functional genomics and breeding programs.Fortunately,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas)technologies,by allowing multiplex and precise genome engineering,have become valuable genome-editing tools and opened up new avenues for biotechnology.Here,we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing.We also summarize the application of CRISPR/Cas technologies to Brassica crops for trait improvements.Finally,we discuss the challenges and future directions of these technologies for comprehensive application in Brassica crops.Ongoing advancement in CRISPR/Cas technologies,in combination with other achievements,will play a significant role in the genetic improvement and molecular breeding of Brassica crops.
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