Evaluation of the diagnostic value of serum-based proteomics for colorectal cancer
作者机构：Department of Respiratory and Critical Care MedicineBeijing Chao-Yang HospitalBeijing Institute of Respiratory MedicineCapital Medical UniversityBeijing 100020China Department of Pancreatic and Gastric SurgeryNational Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing)Interventional Therapy DepartmentPeking University Cancer Hospital and InstituteBeijing 100142China Medical Innovation Research Division of Chinese PLA General HospitalBeijing 100853China
出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)
年 卷 期：2022年第14卷第8期
页 面：1562-1573页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by National Key Research and Development Program of China,No.2020YFC2002700 CAMS Initiative for Innovative Medicine,No.2016-I2M-1-007 National Natural Science Foundation of China,No.81972010,and No.81500003
主 题：Colorectal cancer Colorectal polyps Serum Proteomics Diagnostic value
摘 要：BACKGROUND Colorectal cancer(CRC)is a highly malignant cancer with a high incidence and mortality in *** is urgent to find a diagnostic marker with higher sensitivity and specificity than the traditional approaches for CRC *** To provide new ideas for the diagnosis of CRC based on serum *** Specimens from 83 healthy people,62 colon polyp(CRP)patients,and 101 CRC patients were analyzed by matrix-assisted laser desorption/ionization time-offlight mass *** diagnostic value of the profiles of differentially expressed proteins was then *** Compared with the healthy control group,CRC patients had elevated expression of 5 proteins and reduced expression of 14 *** area under the curve(AUC)for a differentially expressed protein with a mass-to-charge ratio of 2022.34 was the largest;the AUC was 0.843,which was higher than the AUC of 0.717 observed with carcinoembryonic antigen(CEA),and the sensitivity and specificity of this identified marker were 75.3%and 79.5%,*** cross-validation,the accuracy of diagnosis using levels of this differentially expressed protein was 82.37%.Compared with the CRP group,the expression of 3 proteins in the serum of CRC patients was elevated and 11 proteins were expressed at reduced *** possessing mass-to-charge ratio values of 2899.38 and 877.3 were selected to establish a classification tree *** results showed that the accuracy of CRC diagnosis was 89.5%,the accuracy of CRP diagnosis was 81.6%,and the overall accuracy of this approach was 86.3%.The overall sensitivity and specificity of diagnosis using the proteomics approach were 81.8%and 66.75%,*** sensitivities and specificities of diagnoses based on CEA and carbohydrate antigen 19-9 expression were 55.6%and 91.3%and 65.4%and 65.2%,*** We demonstrated that serum proteomics may be helpful for the detection of CRC,and it may assist clinical practice for CRC diagnosis
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