A polyphenol-modified chitosan hybrid hydrogel with enhanced antimicrobial and antioxidant activities for rapid healing of diabetic wounds
作者机构：The State Key Laboratory of Polymer Materials EngineeringPolymer Research Institute of Sichuan UniversityChengdu 610065China Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene RegulationSun Yat-Sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Guangxi Engineering Center in Biomedical Materials for Tissue and Organ RegenerationLife Science InstitutesGuangxi Medical UniversityNanning 530021China Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and MinistryGuangxi Medical UniversityNanning 530021China Bioscience and Biomedical Engineering ThrustThe Hong Kong University of Science and Technology(Guangzhou)Guangzhou 511400China Division of Life ScienceThe Hong Kong University of Science and TechnologyHong Kong 999077China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2023年第16卷第1期
页 面：905-916页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 070305[理学-高分子化学与物理] 0703[理学-化学] 0702[理学-物理学]
基 金：This work was supported by the National Natural Science Foundation of China(No.51973243) Fundamental Research Funds for the Central Universities(No.191gzd35) Guangdong Innovative and Entrepreneurial Research Team Program(No.2016ZT06S029) Shenzhen Basic Research Project(No.JCYJ20190807155801657) Key international(regional)cooperative research projects of the National Natural Science Foundation of China(No.5181001045).
主 题：antimicrobial antioxidant diabetic wound hydrogel
摘 要：High oxidative stress injury and bacterial infection are the main challenges that impair wound healing in diabetic patients.Therefore,a hydrogel with enhanced antimicrobial and antioxidant properties was developed for rapid healing of diabetic wounds.In this study,chitosan methacrylate-gallic acid(CSMA-GA)polymer with antioxidant activity,antimicrobial activity,and ultraviolet(UV)-triggered gelling properties was developed as a hydrogel precursor.Meanwhile,amphiphilic Pluronic F127 molecules were used to load hydrophobic chlorhexidine drug molecules to obtain F127/chlorhexidine nanoparticle(NP)with strong antibacterial activity.Subsequently,F127/chlorhexidine NPs were encapsulated in CSMA-GA hydrogel to further enhance its antibacterial activity.The hybrid hydrogel platform(CSMA-GA/F127/chlorhexidine(CMGFC))exhibited high antibacterial efficiency(99.9%)and strong reactive oxygen species(ROS)scavenging ability(80.0%),which effectively protected cells from external oxidative stress(upregulated superoxide dismutase(SOD)and glutathione/oxidized glutathione disulfide(GSH/GSSG)levels and downregulated malondialdehyde(MDA)levels).Moreover,in vivo results proved that the CMGFC hydrogel significantly reduced inflammatory responses(downregulated interleukin-6(IL-6)and upregulated interleukin-10(IL-10)levels),promoted angiogenesis(upregulated vascular endothelial growth factor(VEGF)and platelet endothelial cell adhesion molecule-1(CD 31)levels),and wound healing(enhanced collagen deposition and tissue remodelling).Overall,the CMGFC hydrogel with enhanced antimicrobial and antioxidant properties demonstrated significant potential to enhance diabetic wound healing.
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